plant tissue culture technique could provide sterile and controllable condition in order to assay direct effect of different compounds on plant growth accurately. in this study, the effects of aqueous extracts prepared from roots and shoots of goosefoot (chenopodium album l.), redroot pigweed (amaranthus retroflexus l.), fennel (foeniculum vulgare), and wormwood (artemisia absinthium l.) were e...

Trehalose, an a,a-diglucoside, induced a rapid blackening and death of shoot tips of Cuseuta reflexa (dodder) cultured in vitro. The onset of toxic symptom was delayed if any of the several sugars which support the in vitro growth of Cuscuta was supplied with trehalose. The rate of trehalose uptake or its accumulation in the tissue was not affected by sugar cofeeding. The levels of total and re...

A novel combination of plant growth regulators comprising indole-3-butyric acid (IBA), 6-benzylaminopurine (BA) and gibberellic acid (GA3) in Murashige and Skoog basal medium has been formulated for in vitro induction of both shoot and root in one culture using cotyledonary node explants of guar, (Cyamopsis tetragonoloba). Highest percentages of shoot (92%) and root (80%) induction were obtaine...

Pigeonpea is an important grain legume, however the genetic transformation of pigeonpea has been very challenging to date. In the present study, we describe a simple protocol for plantlet regeneration via multiple shoot induction from 12-16 day old immature embryos of pigeonpea cultivar ICPL 87 which can be used for efficient transformation. By optimizing the culture medium, we have achieved or...

Two separate experiments in solution culture were conducted simultaneously under saline conditions with varying external K:Na ratios. Shoot and root yield, their composition and selectivity of Kover Na under all set of conditions were calculated for NIAB-6 (salt tolerant) and IR 1561 (salt sensitive). Salinity decreased shoot and root yield drastically but different ratios of K:Na and Ca:Na did...

A complete protocol for micropropagation of Musa sapientum using shoot meristems was developed. Multiple shoots were induced in vitro from shoot meristems. Murashige and Skoog’s medium supplemented with BAP and NAA (3.0 + 0.2 mg/l, respectively) was found to be most suitable combination. Further multiplication of shoots required habituation of cultures up to 3 passages of 21 days each on the sa...

Nodal explants bearing a single axillary bud, from three cultivars of cultivated grape, viz., `Thompson seedless', `Sonaka' and `Tas-e-Ganesh', were used to initiate shoot cultures on G16 medium containing adenine sulphate, monobasic sodium phosphate, BAP and NAA. Each shoot, developed from an axillary bud, produced a tuft of multiple shoots on a medium containing BAP, calcium pantothenate, mon...

in vitro multiple shoot regeneration of solanum nigrum l., an indian medicinal plant was accomplished on ms medium utilizing shoot tip and nodal explants. direct multiple shoots differentiated within 6 weeks when explants were cultured on ms medium containing bap (1.0-5.0 mg/l) and kin (1.0-5.0 mg/l) individually. among various concentrations of cytokinins tested, maximum number of multiple sho...

Cecropia glaziovii is a tree with used in Brazilian popular medicine. Methods allowing the clonal propagation of this species are of great interest for superior genotype multiplication and perpetuation. For this reason, we examined the effect of different culture media and different types of explants on adventitious shoot regeneration from callus and buds of C. glaziovii. Leaves, petioles and s...