two experiments were performed for in vitro establishment, proliferation and shoot growth in axillary bud explants of sebri pear cultivar. in the first experiment, the effect of different concentrations of iba(indole-3-butyric acid) on explants’ establishment was evaluated. in a second experiment, the influence of several combinations of pgrs (plant growth regulators)on shoot proliferation and ...

A rapid in vitro propagation system has been established from mature shoot tip and nodal segments of ahighly valuable medicinal plant Alternanthera sessilis (L.). The explants were cultured on Murashige andSkoog’s medium augmented with different concentrations and combinations of plant growth regulators forshoot bud initiation and multiplications. For shoot tip, highest freque...

Dog rose (Rosa canina) has been using as a rootstock for ornamental roses also it is one of the medicinal plants. It can be propagated under in vitro conditions. After removing chilling requirement of buds, axillary wood buds of dog rose were cut in three nodal positions (lower, middle and terminal) on the stem and then explants after decontamination, cultured on Murashige and Skoog medium (MS ...

abstract in vitro leaf explants of gf677 hybrid rootstock and rabi cultivar were placed on mql medium supplemented with bap at 0, 1, 2, 3 and 4 mg/l and naa at 0, 0.15 and 0.3 mg/l concentrations. the results revealed that bap, naa, genotype and their interactions had significant effect on both percentage and mean number of the regenerated shoots. for gf677, the highest percentage of shoot rege...

parsi zira, bunium persicum (boiss.) b. fedtsch., or black zira, a member of the umbellifera family, is one of the most important medicinal species with high economic value. generally, there is limited information about in vitro culture of bunium persicum. in this study, the effect of various media (ms, b5) and combinations of plant growth regulators, naa and 2,4-d alone or together with kin, o...

Direct regenerations by using mature cotyledonary node as a explants has been shown to be time-saving and convenient strategy for micropropagation of soybean. So we have evaluated regeneration protocol through single shoot using cotyledonary node as a rapid and efficient protocol for two soybean cultivars and one mutant line. Cotyledonary nodes explants obtained from 7-days-old in vitro</em...

shoot tips from actively growing, greenhouse maintained plants of kalanchoe blossfeldiana were cultured in vitro for shoot proliferation and root initiation on murashige and skoog (ms) basal medium supplemented with naa and ba, both in concentrations of 0.00, 0.50, 1.00 and 2.00 mg l-1. results showed that the maximum plantlets height (7.012 cm), node number (4.516), root number (8.860) and roo...

Context: It is necessary to focus on the importance of adopting micropropagation technique for mass propagation of the plantlets in commercial scale as well as conservation and distribution of germplasm. Objective: The present investigation has been designed with a view to establishing protocol of in vitro regeneration of medicinal plant species i,e., Vitex nigundo L (Verbenaceae). Materials an...

In the present investigation, a micropropagation protocol has been developed for Vanilla borneensis Rolfe – critically endangered orchid through multiple shoot regeneration. Through in vitro regeneration from both nodal and tip explants, maximum (100%) induction was observed. The minimum time required bud observed (5–7 days) on medium supplemented with BAP (4.44 mM) + KIN (2.32 as compared to e...