Nodal explants bearing a single axillary bud, from three cultivars of cultivated grape, viz., `Thompson seedless', `Sonaka' and `Tas-e-Ganesh', were used to initiate shoot cultures on G16 medium containing adenine sulphate, monobasic sodium phosphate, BAP and NAA. Each shoot, developed from an axillary bud, produced a tuft of multiple shoots on a medium containing BAP, calcium pantothenate, mon...

Excised shoots and cell-free extracts prepared from Wedgwood iris (Iris hollandica Hoog. "Wedgwood") shoots metabolized (14)C-labeled mevalonic acid (MVA). By using cell-free extracts, the (14)C from MVA-1-(14)C was recovered as (14)CO(2), while that from MVA-2-(14)C was recovered as neutral terpenes, acid-hydrolyzable terpenes, or (14)CO(2). Also, under optimal incubation conditions, 12.8 nano...

Quercetin is known to possess antimicrobial activity against bacteria, fungi, and viruses. The activity of this flavonoid against phytoplasmas, non-cultivable plant pathogenic bacteria that cause numerous plant diseases, has never been examined before. The aim of this research was to examine the effect of different concentrations of quercetin (10 mM, 100 mM and 1 mM) on 'Candidatus Phytoplasma ...

Polyploid plantlets, including triploid, tetraploid, and mixoploid, were induced from the European pear (Pyrus communis L.) cultivar ‘Fertility’ by in vitro colchicine treatment of leaf explants. The leaf explants were incubated in 0.4% (w/v) colchicine for 24, 48, or 72 h, then transferred to adventitious shoot-induction medium. Regenerated shoots were pre-selected according to their morpholog...

The frequency of shoot regeneration and multiplication of P. harmala was influenced by the type of explant and kind and concentration of hormones. Of the various seedling explants, cotyledonary node exhibited maximum shoot regeneration frequency from axillary region on MS medium supplemented with 5 microM BAP. Addition of 0.1 microM NAA enhanced the efficacy of BAP for multiple shoot regenerati...

Withania somnifera (L.) Dunal is high demanding valuable medicinal plants which have pharmaceuticals properties, known for its with anolides. Nodal segment explants of W. somnifera when cultured on MS (Murashige and skoog’s, 1969) medium horizontally containing different concentration(1-3mg/l) of 6-benzylaminopurine (BAP), kinetin (Kn) and thidiazuron (TDZ) produced multiple shoots on both axil...

An efficient in vitro regeneration protocol has been developed from shoot tips of Drosera burmannii Vahl., a carnivorous plant of north-east India. Various plant growth regulators were used to study their efficacy in the induction of multiple shoots and roots. Of the various treatments, the maximum number of shoots (28.8 ± 1.5) and roots (9.7 ± 0.6) was observed in one-fourth strength standard ...

The dissipation behavior of octachlorodipropyl ether (OCDPE) residues in fresh tea shoots and in tea prepared under field conditions was investigated, and the transfer of residues from brewed tea to tea infusion was determined. OCDPE levels in tea shoots, prepared tea, tea infusion, and spent tea leaves were determined using a sensitive and simple method. The dissipation of OCDPE is fairly slow...

Roots of plantlets of Garcinia indica when cultured for long time on half strength MS medium supplemented with BAP (0.44-2.22 microM) showed production of de novo shoots. Roots attached to mother plant showed more number of shoots, while excised root segments produced lesser shoots. Shoots (0.5-0.8 cm) were transferred to elongation medium consisting of Woody Plant Medium (WPM) supplemented wit...