Bacterial resistance is a serious threat to human health. The production of β-lactamase, which inactivates β-lactams is most common cause of resistance to the β-lactam antibiotics. The Class A enzymes are most frequently encountered among the four β-lactamases in the clinic isolates. Mutations in class A β-lactamases play a crucial role in substrate and inhibitor specificity. SHV and TEM type a...

BACKGROUND & OBJECTIVES Extended spectrum beta lactamases (ESBLs) have been observed in virtually all the species of family Enterobacteriaceae. The enzymes are predominantly plasmid mediated and are derived from broad-spectrum beta lactamase TEM-1, TEM-2 or SHV-1 by a limited number of mutations. This study was undertaken to characterize ESBL producers among Escherichia coli and Klebsiella pneu...

OBJECTIVES To investigate the occurrence and the diversity of Ambler class A ESBLs among Enterobacteriaceae from different Portuguese clinical settings over a 2 year period (2002-04). METHODS One hundred and nine extended-spectrum beta-lactamase (ESBL)-producing Enterobacteriaceae isolates from five geographically distant health institutions in Portugal were studied. ESBLs were characterized ...

A new description of the exact completion Cex/reg of a regular category C is given, using a certain topos Shv(C) of sheaves on C; the exact completion is then constructed as the closure of C in Shv(C) under finite limits and coequalizers of equivalence relations. An infinitary generalization is proved, and the classical description of the exact completion is derived.

A new description of the exact completion C ex/reg of a regular category C is given, using a certain topos Shv(C) of sheaves on C; the exact completion is then constructed as the closure of C in Shv(C) under nite limits and coequalizers of equivalence relations. An innnitary generalization is proved, and the classical description of the exact completion is derived.

To determine the prevalence and genotypes of extended-spectrum beta-lactamases (ESBLs) among clinical isolates of Klebsiella pneumoniae and Escherichia coli, we performed antibiotic susceptibility testing, pI determination, induction testing, transconjugation, and DNA sequencing analysis. Among the 509 isolates collected from 13 university hospitals in Korea, 39.2% produced ESBLs. ESBL-producin...

One hundred four Enterobacter isolates were tested by standard CLSI disk diffusion methods for detecting extended-spectrum beta-lactamases (ESBLs) and with cefepime-clavulanate disk combinations. SHV-12 was produced by 8.7% of isolates. The cefepime-clavulanate combination provided 88% sensitivity and 91% specificity for the detection of SHV-12 ESBL.

BACKGROUND & OBJECTIVE Phenotyping is commonly used for detection of extended spectrum beta lactamase (ESBL) production in gram-negative isolates. ESBLs are mainly coded for by three important genes, namely bla(TEM), bla(SHV) and bla(CTX-M). In this study we used a multiplex PCR as a rapid method to identify two common genes (bla(CTX-M) & bla(SHV)) responsible for extended spectrum beta lactama...

The preferred embodiment of dual linear polarization technology on the WSR-88D weather radars is the mode whereby horizontal (H) and vertical (V) polarizations are transmitted and received simultaneously (Doviak et al. 2000). This mode is sometimes referred to as “hybrid” (Wang et al. 2006); to shorten notation we label it SHV (Simultaneous Horizontal and Vertical). The USA National Weather Ser...