We present a miniaturized pull-down method for the detection of protein-protein interactions using standard affinity chromatography reagents. Binding events between different proteins, which are color-coded with quantum dots (QDs), are visualized on single affinity chromatography beads by fluorescence microscopy. The use of QDs for single molecule detection allows the simultaneous analysis of m...

Electron-phonon interactions of free charge-carriers in doped pi-conjugated polymers are conceptually described by 1-dimensional (1D) delocalization. Thereby, polaronic transitions fit the 1D-Froehlich model in quasi-confined chains. However, recent developments in conjugated polymers have diversified the backbones to become elaborate heterocylcic macromolecules. Their complexity makes it diffi...

The paper concerns the modelling of masonry elements strengthened through Textile Reinforced Mortar (TRM), a near surface system made fiber-based grids or textiles embedded in mortar layers. Recently, author, focusing on mechanical characterization TRM composites, developed detailed level approach by using free, open-source Finite-Element code OOFEM, for simulation experimental tests coupons (p...

Abstract Consider a Shimura variety of Hodge type admitting smooth integral model S at an odd prime $$p\ge 5$$ p ≥ 5 . its perfectoid cover $$S^{\text {ad}}(p^\infty )$$ S ad (</mml:...

PURPOSE To identify proteins that physically interact with Pax-6, a paired domain- and homeodomain (HD)-containing transcription factor that is a key regulator of eye development. METHODS Protein-protein interactions involving Pax-6, TATA-box-binding protein (TPB), and retinoblastoma protein were studied using affinity chromatography with Pax-6 as ligand, glutathione-S-transferase (GST) pull-...

Use of Engineered Cementitious Composites (ECC) has been proven to enhance structural fatigue resistance and reduce the use-phase emissions for transportation infrastructure. Carbonation curing offers an opportunity embodied carbon ECC via direct CO2 sequestration. In this study, impact carbonation on ECC’s was examined. uptake, static flexural behavior, performance, single fiber pull-out behav...

Composite fiber reinforcement is one such proficient material that substitutes for useful applications conventional and polyester blends where little weight thus less energy conservation required. This study highlighted the importance of recently discovered Agave Angustifolia marginated (AAM) fibers extracted by a manual process from AAM plants. paper observes various properties mechanical mois...