نتایج جستجو برای: single strand conformational polymorphism

تعداد نتایج: 995443  

Journal: :iranian journal of biotechnology 2009
roohallah barzehkar abdolreza salehi frouzandeh mahjoubi

leptin (lep), the expression product of the obese gene produced primarily in the adipose tissue, is related to feed intake, growth and lipid metabolism. the aim of this study was to study the possible association between polymorphism of the lep gene with growth and carcass traits among three iranian sheep breeds of the shal, zandi and zel varieties. a total of 180 purebred animals of the shal, ...

Journal: :Journal of Korean Medical Science 2003
Won Sang Park Yong Gu Cho Jik Young Park Chang Jae Kim Jong Heun Lee Hong Sug Kim Jong Woo Lee Young Hwa Song Cho Hyun Park Yong Kyu Park Su Young Kim Suk Woo Nam Sug Hyung Lee Nam Jin Yoo Jung Young Lee

Recently, the -160 C/A polymorphism, located within the regulatory region of Ecadherin promoter, has been shown to influence E-cadherin transcription by altering transcription factor binding. We examined the effect of this polymorphism on risk of gastric cancer and on histological classification of intestinal- and diffuse-type gastric cancer in 146 normal healthy individuals and 292 Korean gast...

Journal: :Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologicas 2006
M Houshmand T Mahmoudi M Shafa Shariat Panahi Y Seyedena S Saber M Ataei

Leber's hereditary optic neuropathy (LHON) is a maternally inherited form of retinal ganglion cell degeneration leading to optic atrophy in young adults. Several mutations in different genes can cause LHON (heterogeneity). The ND6 gene is one of the mitochondrial genes that encodes subunit 6 of complex I of the respiratory chain. This gene is a hot spot gene. Fourteen Persian LHON patients were...

2015
Pooja H. Gupta Nirmal A. Patel D. N. Rank C. G. Joshi

AIM An attempt has been made to study the toll-like receptors 4 (TLR4) gene polymorphism from cattle DNA to correlate with mastitis cows. MATERIALS AND METHODS In present investigation, two fragments of TLR4 gene named T4CRBR1 and T4CRBR2 of a 316 bp and 382 bp were amplified by polymerase chain reaction (PCR), respectively from Kankrej (22) and Triple cross (24) cattle. The genetic polymorph...

2017
Gang Sheng Tasos Gogakos Jiuyu Wang Hongtu Zhao Artem Serganov Stefan Juranek Thomas Tuschl Dinshaw J. Patel Yanli Wang

We have undertaken a systematic structural study of Thermus thermophilus Argonaute (TtAgo) ternary complexes containing single-base bulges positioned either within the seed segment of the guide or target strands and at the cleavage site. Our studies establish that single-base bulges 7T8, 5A6 and 4A5 on the guide strand are stacked-into the duplex, with conformational changes localized to the bu...

Ali Javadmanesh Mahdi Soltani Mohammad Reza Nassiry, Mojtaba Tahmoorespour Saheb Foroutani Far

Association of genetic polymorphism in the calpastatin (CAST) gene with average daily gain was examined in Iranian purebred Kurdi sheep. The genotypes for CAST were determined by the PCR-SSCP method. Blood samples were collected from 84 purebred Kurdi sheep belonging to the Kurdi Breeding Station located in the Khorasan province, north-east of Iran. Extraction of genomic DNA was based on the Gu...

Journal: :Journal of biochemistry and molecular biology 2002
Hoi-Shan Kwan Hai-Lou Xu

From fruiting bodies of L. edodes strain L-54, single-spore isolates (SSIs) were collected. Two parental types of L-54 were regenerated via monokaryotization. By means of random-amplified polymorphic DNA (RAPD), DNA samples from L-54, its two parental types, and 32 SSIs were amplified with arbitrary primers. Dedikaryotization was demonstrated, and 91 RAPD-based molecular markers were generated....

Journal: :journal of cell and molecular research 0
fatemeh nasernakhaei mohammad reza rahiminejad hojjatollah saeidi manoochehr tavassoli

as a case study, the genotypic polymorphism of the iranian material of diploid triticum was screened based on internal transcribed spacer 2 (its2) of the ribosomal dna (rdna) using single strand conformation polymorphism (sscp) analysis. this is a simple and cost effective technique for genotyping and investigating the allelic polymorphism among plant populations. performing sscp analysis among...

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