BACKGROUND & OBJECTIVE Rubella virus (RV) is one of the leading causes of childhood blindness in India. In this study we applied an optimized nested reverse transcription polymerase chain reaction (nRT-PCR) to detect RV in clinical specimens. METHODS nRT-PCR was optimized using total RNA extracted from standard strain of RV using nested sets of primers specific for E1 open reading frame. nRT-...

The Short Time Exposure (STE) test is a simple and easy-to-perform in vitro eye irritation test, that uses the viability of SIRC cells (a rabbit corneal cell line) treated for five minutes as the endpoint. In this study, our goal was to define the applicability domain of the STE test, based on the results obtained with a set of 113 substances. To achieve this goal, chemicals were selected to re...

در بحث امدادرسانی به مصدومان بلایای طبیعی و غیرطبیعی، هدف اصلی یک زنجیره این است که اقلام موردنیاز مانند آب مواد غذایی، دارویی، پناهگاه سایر ملزومات سریع‌ترین زمان ممکن در­دسترس قرار بگیرد تا از تعداد مرگ‌ومیر ناشی وقوع بلایا حد امکان کاسته شود؛ بنابراین طراحی توسعه اجرایی­کردن می‌تواند نقش مهم بزرگی دست­‌یافتن پاسخ مناسب ایفا کند. بارزترین تفاوت‌ها برخورد با ، غیرقابل‌پیش‌بینی‌­بودن تقاضا نظر ...

AIM To determine the induced corneal astigmatism by measuring the changes in manual keratometry and computerised corneal videokeratoscopy up to 1 year following small flap trabeculectomy (microtrabeculectomy). METHOD A prospective study of a case series of small flap trabeculectomy procedures performed at the 90 degree meridian on 16 eyes of 16 patients, all followed to 1 year postoperatively...

Short time exposure (STE) test using rabbit corneal cell line (SIRC) cells was developed as an alternative eye irritation test. STE test uses relative viability as the endpoint after cells are exposed to the test material at constant concentrations for 5 min. In this inter-laboratory study with 3 laboratories, 44 chemicals with a wide range of classes were evaluated for the transferability, bet...

Rabbi t antisera raised against rabbit corneal (SIRC) cells infected with xenotropic murine leukemia virus (MuLV) 1 contain antibodies specific for antigens related to the major glycoproteins (gp70) o f the xenotropic M u L V envelope (1). These antigens appear to be normal constituents of the cell surface o f lymphoeytes of all inbred mice and have been termed XenCSA-xenotropic M u L V envelop...

Plinia cauliflora (Mart.) Kausel, widespread in South America, has edible fruits, and its bark is commonly used against diarrhea and other disorders, on account of its astringency. Because diarrhea is still one of the most important causes of illness and death among children in developing countries, where the population turns to traditional medicine for its treatment, the present study determin...

In recent years, multiple drug resistance has been developed due to indiscriminate use of existing drugs in the treatment of infectious diseases. The major thrust is to establish alternative antimicrobial agent in order to treat microbial infections with less or no toxicity and less or negligible side effects. The herbal medicines have shown potential to overcome the limitation associated with ...

Claviceps purpurea is an important food contaminant and well known for the production of the toxic ergot alkaloids. Apart from that, little is known about its secondary metabolism and not all toxic substances going along with the food contamination with Claviceps are known yet. We explored the metabolite profile of a gene cluster in C. purpurea with a high homology to gene clusters, which are r...

PURPOSE This study evaluated the cytotoxicity of five prostaglandin analog ophthalmic solutions on four ocular surface cell lines, ie, Chang (human conjunctiva), SIRC (rabbit cornea), RC-1 (rabbit cornea), and BCE C/D-1b (bovine cornea). METHODS Cell viability was measured by neutral red and MTT assays in cells treated for 10, 30, or 60 minutes with various doses of prostaglandins (undiluted,...