Propionibacterium acnes is an important member of the skin microbiome. The bacterium can initiate signalling events and changes in cellular properties in keratinocytes. The aim of this study was to analyse the effect of the bacterium on an immortalized human keratinocyte cell line. The results show that various P. acnes strains affect the cell-growth properties of these cells differentially, in...

We studied the expression of the male-specific mH antigen H-Y on cultured human skin cells by investigating susceptibility to H-Y-specific cytolysis using conventional class I-restricted CTL clones in a modified cell-mediated cytotoxicity assay. In contrast to what was found in the rodent system, we observed H-Y-specific lysis of human male keratinocytes. Susceptibility for H-Y-specific lysis w...

gate and her group reviewed bioprocessing of human fetal cells for tissue engineering of skin. Wiegand and Hipler evaluate the biocompatibility and cytotoxicity using keratinocyte and fibroblast cultures. The importance of tissue engineering is covered by 2 chapters by Macri and Clark as well as by Auger et al. Immunological aspects and tissue engineering in the context of in vitro irritation m...

Apoptosis is a process of programmed cell death that maintains homeostasis of the skin. Apoptotic cell death regulates keratinocyte proliferation and formation of stratum corneum. The process by which keratinocytes undergo apoptosis is a multistep program mediated by binding of specific death ligands to death receptors or by the release of effector cell granules. Dysfunctional apoptosis has an ...

in a study of 310 patients with suspected dermatophytic lesions, 110 cases of dermatophytosis, were diagnosed by direct smear in 10% koh and culture by two methods, skin stripping with use of adhesive tape and scrapping with scalpel blade conventional technique). out of 65 positive cultures, in 33 cases, growth was obtained by methods (scraping and stripping), 23 by the conventional technique a...

Copper is an essential mineral and plays important roles in skin growth and activity. Copper delivery through skin can provide beneficial effects but its potential to induce skin irritation reactions is often overlooked. Data on dermal toxicity caused by copper compounds is scant. Some recognized in vitro skin toxicity methods are unsuitable for all metal compounds. Here, we employ a keratinocy...

Biofilm-protected microbial infections in skin are a serious health risk that remains to be adequately addressed. The lack of progress in developing effective treatment strategies is largely due to the transport barriers posed by the stratum corneum of the skin and the biofilm. In this work, we report on the use of Ionic Liquids (ILs) for biofilm disruption and enhanced antibiotic delivery acro...

BACKGROUND The preference for a fairer skin-tone has become a common trend among both men and women around the world. In this study, seaweeds Sargassum polycystum and Padina tenuis were investigated for their in vitro and in vivo potentials in working as skin whitening agents. Seaweed has been used as a revolutionary skin repairing agent in both traditional and modern preparations. The high ant...

Sun exposure and cigarette smoke, studied separately, are two well-known environmental factors that accelerate skin aging. However, their combined effects has been poorly characterized. Some studies have determined smoke components can accumulate in the by contact or systemic effect after inhalation. Moreover, it is well-established solar rays penetrate epidermis dermis. Those could thus intera...

Antimicrobial peptides (AMPs) from the dermaseptin and phylloseptin families were isolated from the skin secretion of Phyllomedusa nordestina, a recently described amphibian species from Northeastern Brazil. One dermaseptin and three phylloseptins were chosen for solid phase peptide synthesis. The antiprotozoal and antimicrobial activities of the synthetic peptides were determined, as well as t...