Fluoroquinolones acting equally through DNA gyrase and topoisomerase IV in vivo are considered desirable in requiring two target mutations for emergence of resistant bacteria. To investigate this idea, we have studied the response of Staphylococcus aureus RN4220 to stepwise challenge with sparfloxacin, a known dual-target agent, and with NSFQ-105, a more potent sulfanilyl fluoroquinolone that b...

Pseudomonas aeruginosa is an opportunistic pathogen, armed with multiple resistance mechanisms that render it resistant to many antibiotics. One such mechanism is the ability to form biofilms. A previous study assessed the viability of P. aeruginosa cells within biofilms after physical disruption and antibiotic treatment, and found that there was no difference in cell viability despite physical...