development of a stool antigen immunoassay to detect helicobacter pylori infection requires monoclonal antibody against the specific antigen. alkylhydroperoxide reductase (ahpc) of helicobacter pylori has been described as a specific and unique enzyme for h. pylori and therefore, both h. pylori ahpc and anti-ahpc could be useful in the development of serologic and stool antigen tests, for detec...

A protease produced by Vibrio mimicus was purified to apparent homogeneity by ammonium sulfate fractionation and successive column chromatography on Sephacryl S-100 and Mono Q Monobeads. The sodium dodecyl sulfate-polyacrylamide gel electrophoresis (PAGE) of the final preparation of the enzyme revealed the homogeneity of the purified enzyme. Conventional PAGE showed that the purified protease m...

(J. Am. Soc. Nephrol. 1992; 3:113-1 15) washed gently in PBS and solubibized in 1 mL/wehl of 0. 1 % sodium dodecyb sulfate (BioRad, Richmond, CA), and the radioactivity was estimated with a gamma counter (LKB-Bnomma, Sweden). To study the effects of fixation, the lectin binding was measured In cells fixed in 95% ethanol. Because the fixed cells could not be solubihizcd completely in sodium dode...

a simple hydrothermal method was designed for the synthesis of the nanostructure barium phosphonate in presence of cetyltrimethylammonium bromide (ctab) or sodium dodecyl sulfate (sds) as surfactant. ft-ir, edax, xrf, xrd, sem were used to characterize this material. effect of solvent on morphology complex was discussed. thermogr avimetric analysis (tga) shows the existence of porosity and stab...

Fructan-hydrolyzing enzyme from Streptococcus salivarius KTA-19 isolated from human dental plaque was investigated. The enzyme was purified by ammonium sulfate precipitation, acetone fractionation, and column chromatography on Bio-Gel and DEAE-cellulose. The purified enzyme showed a single band on sodium dodecyl sulfate-polyacrylamide gel electrophoresis and isoelectric focusing. Its molecular ...

Abstract A novel nitrogen-doped carbon quantum dots (N-CDs) were prepared by the microwave irradiation method. The fluorescence quenching effect of Co(II) on N-CDs was studied in sodium dodecyl sulfate (SDS) medium and sensitized SDS. linear range calibration curve for determination 0.17µg/mL-11.8µg/mL limit detection 0.052µg/mL. method has been applied samples with satisfactory results.

Surfactants are chemicals that adsorb to the interface between a solution and another phase (gases or solids) self-assemble molecular clusters known as micelles in (water oil phase). Measurements of surface tension sodium dodecyl sulfate (SDS) distilled water at 298.15K reported. The increase concentration then decreased 298.15K. Critical micelle (CMC) with decrease tension. graphs versus were ...

The conformations of synthetic peptides corresponding to signal sequences of chicken lysozyme and Escherichia coli proteins alkaline phosphatase and lipoprotein (wild-type) and their "variants" with a charged amino acid in the hydrophobic region, have been studied by circular dichroism spectroscopy in trifluoroethanol and micelles of sodium dodecyl sulfate, Brij 35, and sodium deoxycholate. In ...

fractional flow analysis confirms the advantages of surfactant alternative gas injection (sag) in enhanced oil recovery, but an adsorption phenomenon that has been affected by several factors, weakens the effectiveness of sag injection. in this study, the effects of sacrificial agent, gas phase, and surfactant concentration on adsorption density on silica mineral were investigated by static and...

Antibodies to group A streptococcal carbohydrate, demonstrable by radioimmunoassay, were elicited in Pacific hagfish, Eptatretus stoutii, after prolonged immunization with whole cell vaccine. This hagfish antibody, isolated by ion-exchange chromatography of immune serum, exhibited a single precipitin line after immunoelectrophoresis against rabbit antiserum to hagfish serum. The isolated antibo...