A noncollagenous protein, which is a major component of the extracellular matrix of third trimester fetal epiphyseal cartilage, has been isolated and characterized. On sodium dodecyl sulfate-polyacrylamide gel electrophoresis under reducing conditions, the protein exists in the form of its 35,000 molecular weight subunit. Under nonreducing conditions, the protein exists as a 69,000 molecular we...

Hemolysins purified from non-O1 Vibrio cholerae (non-O1 hemolysin) and a Vibrio cholerae O1, biotype El Tor (El Tor hemolysin) were investigated for their homology. The hemolysins were isolated from the culture supernatant fluids by ammonium sulfate precipitation and gel filtration on Sephadex G-100 columns. The purified hemolysins gave single bands with an identical mobility on conventional po...

A novel neutrophilic metalloprotease was isolated from Pseudomonas sp. DR89 isolate which was identified ina mineral spring in Iran. The enzyme was purified from the isolate to 21-folds in a three-step procedure involving ammonium sulfate precipitation, Q-Sepharose ionic exchange and Sephadex G-100 gel filtrationchromatography. Resuts showed that the enzyme was active at high temper...

A number of enzymes, including amylases, dehydrogenases, and proteases, were shown to be renaturable after polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulfate. Enzyme activity was detected in situ by action on substrates introduced into the gel and subsequent staining of either the product or unreacted substrate. This technique combines the advantage of enzyme identific...

DNA topoisomerase I has been purified from homogenates of mature Xenopus laevis ovaries. The initial stages in purification of the native enzyme employed a rapid series of three chromatographic steps, followed by gel filtration performed in the presence of sodium dodecyl sulfate. Polypeptides that might represent topoisomerase I were identified by specific labeling of the topoisomerase species ...

The yolk precursor protein vitellogenin was isolated from plasma of estrogen-treated Xenopus laevis males, and the yolk platelet proteins, lipovitellin and phosvitin, were isolated from oocytes of females previously stimulated with human chorionic gonadoptropin. The molecular weights of the polypeptide chains of vitellogenin and lipovitellin were determined by electrophoresis on sodium dodecyl ...

Mouse myeloid leukemic M1 cells are induced to differentiate by various differentiation inducers. Activity for inhibition of induction of differentiation of M1 cells (I-factor activity) was detected in conditioned medium of variant M1 cell clones that were resistant to differentiation inducers, and this I-factor activity was shown to be closely associated with resistance of the cells to differe...

this study was conducted to investigate the effects of doses of 25, 50 and 75 kgy of gamma ray (gr) and electron beam (eb) ionizing radiations on ruminal disappearance of amino acids (aas) and protein subunits of canola meal (cm). the nylon bag technique was used for degradability trial. three ruminally fistulated taleshi bulls were used for this aim. the disappearance trends of protein subunit...

We have developed novel protein gel electrophoresis techniques, sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and isoelectric focusing (IEF) in short microchannels (approximately millimeters) that take less than a minute. A photopatterning technique was used to cast in situ crosslinked polyacrylamide gel in a microchannel to perform SDS-PAGE. A fluorescent protein marker ...

Antibodies to group A streptococcal carbohydrate, demonstrable by radioimmunoassay, were elicited in Pacific hagfish, Eptatretus stoutii, after prolonged immunization with whole cell vaccine. This hagfish antibody, isolated by ion-exchange chromatography of immune serum, exhibited a single precipitin line after immunoelectrophoresis against rabbit antiserum to hagfish serum. The isolated antibo...