Characterizing the genetic diversity of microbial copper (Cu) resistance at the community level remains challenging, mainly due to the polymorphism of the core functional gene copA. In this study, a local BLASTN method using a copA database built in this study was developed to recover full-length putative copA sequences from an assembled tailings metagenome; these sequences were then screened f...

Mining tailings as a raw material for preparing autoclaved bricks have attracted much attention. The tailings subjected to milling always have the characteristic of fine granularity. However, the proportion of the fine aggregates in autoclaved bricks was very low using the current gradation formula, which impeded the utilization of the tailings. In this work, a novel model of aggregate gradatio...

In 1985, a tailing dam collapsed in Hunan province (southern China) leading to soil contamination by heavy metals from the tailings waste. Moreover, acid deposition becomes more and more serious in this area. In this context, two forest soils (a red soil and a yellow red soil, typically and commonly found in southern China) were collected from Hunan. The objectives are (i) to determine releases...

Biological nitrogen fixing is an important source of nitrogen input in the natural ecological restoration of mine wastelands. The diversity of nifH genes in tailings samples under different plant communities in Yangshanchong and Tongguanshan wastelands in Tongling, was analyzed using polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE) approach. The nitrogen-fixing micro...

(1) Boudens R, Reid T, VanMensel D, Prakasan MRS, Ciborowski JJ, Weisener CG. Biophysicochemical effects of gamma irradiation treatment for naphthenic acids in oil sands fluid fine tailings. Sci Total Environ 2016 Jan 1;539:114-24. Abstract: Naphthenic acids (NAs) are persistent compounds that are components of most petroleum, including those found in the Athabasca oil sands. Their presence in ...

A detailed investigation of the mineralogy of As in the tailings of the Rabbit Lake uranium ore processing facility was conducted. The milling/ ore extraction process was sampled at three different locations to obtain information about when, where and under what condition secondary As phases form. These samples were compared with four samples of varying As content from the Rabbit Lake in-pit ta...

Rainbow trout were exposed in situ to oil sands-affected waters for 21 d, either with or without an immune stimulation using inactivated Aeromonas salmonicida. Three aquatic systems were utilized for the experiment: a pond containing oil sands tailings capped with approximately 3 m of natural surface water, a second pond where unextracted oil sands materials were deposited in the watershed, and...

South Africa is a mineral-rich country with metals such as gold, copper, and platinum group metals being exploited to a significant extent in the country’s mining history. Mining generates large volumes of tailings, with consequent disposal and environmental problems. By far the most gold that has been mined in South Africa (98%) has come from the Witwatersrand goldfields (Messner, 1991). The g...

Draft genome sequences of two Campylobacterales (Sulfurospirillum sp. strain SCADC and Sulfuricurvum sp. strain MLSB [Mildred Lake Settling Basin]) were obtained by taxonomic binning of metagenomes originating from an oil sands tailings pond. Both genomes contain soxABXYZ genes involved in sulfur oxidation, highlighting their potential roles in sulfur cycling in oil sands tailings ponds.

The extent of arsenic (As) and metal accumulation in fronds of the As hyperaccumulator Pteris vittata (Chinese brake fern) and in leaves of Cyperus involucratus, which grow on the surface of an old flotation tailings pond in the Zambian Copperbelt province, was studied. The tailings consist of two types of material with distinct chemical composition: (1) reddish-brown tailings rich in As, iron ...