example, Rao-Arelli et al. (1992b) showed that several isolates of H. glycines were initially classified as one Soybean cyst nematode (SCN) (Heterodera glycines Ichinohe), race by this scheme. However, when later tested with the most destructive pest of soybean [Glycine max (L.) Merrill], is estimated to be responsible for almost nine million megagrams in resistant genotypes other than the stan...

Domesticated soybean [Glycine max (L.) Merr.] is a major crop with an established ancestral relationship to wild soybean (Glycine soja Sieb. & Zucc.) native to Asia. Soybean genetic diversity can be assessed at different levels by identification of polymorphic alleles at genetic loci, in either the plastid or nuclear genomes. The objective of this study was to evaluate genetic diversity based o...

A real-time PCR assay was developed to quantify in soil the fungus Hirsutella minnesotensis, an important parasite of secondary-stage juvenile (J2) of the soybean cyst nematode. A primer pair 50-GGGAGGCCCGGTGGA-30 and 50-TGATCCGAGGTCAACTTCTGAA-30 and a TaqMan probe 50-CGTCCGCCGTAAAACGCCCAAC-30 were designed based on the sequence of the ITS region of the rRNA gene. The primers were highly specie...