The present report was designed to study the macrophage role and their association with percentage of spermatozoa showing DNA native, morphologically normal sperm and occurrence of antibody-coated spermatozoa. Human sperm samples from 31 patients were used in this study. All samples were evaluated for standard semen parameters according to World Health Organization criteria. The sperm heads wit...

BACKGROUND The influence of ejaculatory abstinence (EA) on semen parameters and subsequent reproductive outcome is still debatable; hence understanding the impact of EA on sperm structural and functional integrity may provide a valuable information on predicting successful clinical outcome. OBJECTIVE To understand the influence of EA on sperm chromatin maturity, integrity, longevity and globa...

BACKGROUND: Multiple studies have shown sperm concentration and count decline in many countries, however, authors conclusions were inconsistent. These types of not conducted Russia so far.
 AIM: To evaluate analyze semen parameters among men attending a fertility center St. Petersburg between 2016 2022.
 MATERIALS AND METHODS: In retrospective study 14234 consequent analyses performed...

Living cells, including spermatozoa, stored under aerobic conditions require oxygen to support their normal metabolism. Excessive levels of metabolites (reactive oxygen species; ROS) can cause cell damage or lipid peroxidation. The ROS and lipid peroxidation can be detected during preparation and cryopreservation of mammalian spermatozoa. The negative effects of ROS can be partially ameliorated...

YOUTHEN is a polyherbal formulation. The present was designed to establish the effect of YOUTHEN on androgenic and reproductive parameters of male albino rats. YOUTHEN at lower dose stimulated the haemopoietic function significantly. Higher dose of YOUTHEN significantly enhanced the sperm count, forward motility index and metabolic activity of tech spermatozoa of the experimental animals as com...

Cryopreservation is a technique for storing cells and tissues at very low temperatures the possible usage of stored throughout year. Sperm cell cryopreservation in some species causes decrease sperm quality DNA damage. Inappropriate protocols can cause changes physiology. Mitochondria are organelles that play role producing energy motility. have molecules with small sizes compared to structures...

The aim of current study was to investigate the changes in the quality parameters of Holstein bull semen during the heat stress and the effect of feeding a source of omega-3 fatty acids in this period. Samples were obtained from 19 Holstein bulls during the expected time of heat stress in Iran (June to September 2009). Control group (n=10) were fed a standard concentrate feed while treatment gr...

Pentoxifylline (PF) is used to enhance motility of spermatozoa from infertile human subjects. We have previously shown that 0.45 mM PF improved capacitation of spermatozoa and fertilization of oocytes in vitro in hamsters. The present study was carried out to assess PF-induced changes in motility kinematics of hamster spermatozoa by a computer-aided sperm analyser (CASA) and determine the timin...

Semen samples from 9 sexually mature dogs of different breeds, aged from 1 to 5 years were used. Quality of sperm was evaluated using the following parameters: progressive motility (subjective), then structural and functional integrity of spermal membrane (supravital colouring (eosine/nigrosine) and HOS test). The majority of HOS reactive or swollen spermatozoa was placed in a hypoosmolar solut...

Sperm mediated gene transfer (SMGT) could provide the opportunity to carry out transgenesis on a mass scale using spermatozoa as vectors for exogenous DNA. However, the efficiency of sperm-mediated DNA transfer is still questionable, and the mode of transmission to the egg has not yet been well understood. Our aim was to investigate the capacity of bovine spermatozoa to carry exogenous DNA and ...