Particulate and soluble fractions from leaves of Sorghum, Spinacia (spinach), and Coleus, capable of metabolizing l-phenylalanine to cinnamate or to caffeate, are also able to convert l-and d-phenylalanine to phenylacetate. Since cinnamate and phenylacetate are not effectively separated in commonly used chromatographic solvents, some of the isotropic assays used for phenylalanine ammonia-lyase ...

Incubation of intact spinach (Spinacia oleracea L.) chloroplasts in the presence of (35)SO(4) (2-) resulted in the light-dependent formation of a chloroform-soluble sulfur-containing compound distinct from sulfolipid. We have identified this compound as the most stable form (S(8)) of elemental sulfur (S(0), valence state for S = O) by mass spectrometry. It is possible that elemental sulfur (S(0...

Cyanide inhibited unesterified fatty acid synthesis but stimulated glyceride synthesis from [1-(14)C]acetate when Spinacia oleracea chloroplasts were incubated in basal media. Both unesterified fatty acid and glyceride accumulation were inhibited when chloroplasts were incubated in a diacylglycerol mode. Stimulation of chloroplast fatty acid synthesis by either exogenous coenzyme A or Triton X-...

Acetate concentration in spinach (Spinacia oleracea L.) leaf tissue has been determined by direct measurement in a leaf extract to oe 70 |xm or less. Additional evidence for such low acetate levels came from isotope dilution experiments where the [ l 14C]acetate incorporation into fatty acids was assayed using purified chloroplasts in the presence o f tissue extracts. Pyruvate dehydrogenase com...