The sterility test is generally recognized as a flawed test for its stated purpose. Since the original description in 1932, this test has generated controversy as to its role in product quality testing and in terms of means to improve the assay. As early as 1956 Bryce published an article describing the two critical limitations of this test (1). He put forward that the test was limited in that ...

that the pollen grains had germinated and that the pollen tubes had penetrated the stylar tissue. Demerec's third suggestion remains as a possibility and a fourth may be added which is likewise untested. The failure of gal pollen to fertilize Gals plants may be independent of stylar interaction altogether, and may involve some abnormal reaction within the ovule, such as inability of the pollen ...

Though cytoplasmic male sterility (CMS) in peppers is associated with the orf507 gene, definitive and direct evidence that it directly causes male sterility is still lacking. In this study, differences in histochemical localization of anther cytochrome c oxidase between the pepper CMS line and maintainer line were observed mainly in the tapetal cells and tapetal membrane. Inducible and specific...

The recent detection of a young pulsar powering " the Mouse " , G359.23–0.82, as well as detailed imaging of surrounding nebular X-ray emission, have motivated us to investigate the structural details and polarization characteristics of the radio emission from this axisymmetric source with a supersonic bow shock. Using polarization data at 3.6 and 6cm, we find that the magnetic field wraps arou...

A radiation sterilization dose (RSD) of 25 kGy is deleterious to bone allografts. This study aimed to establish a lower RSD for bone allografts using method 1 of International Standard Organisation 11137.2:2006. This provides a database to select an RSD corresponding to an allograft's bioburden, given that the bioburden's gamma resistance is equal to or less than the standard. This can be verif...

An early event in RNA interference (RNAi) is the cleavage of the initiating double-stranded RNA (dsRNA) to short pieces, 21 to 23 nucleotides in length. Here we describe a null mutation in dicer-1 (dcr-1), a gene proposed to encode the enzyme that generates these short RNAs. We find that dcr-1(-/-) animals have defects in RNAi under some, but not all, conditions. Mutant animals have germ line d...

In a previous paper by Snell and Aebersold' the production in male mice by irradiation with neutrons of an initial period during which small litters are sired, followed by a period of complete sterility, followed, in turn, for the smaller doses, by a resumption of normal fertility, has been described. This paper is concerned with the appearance of sterility and of heredity changes in the descen...

S tcrility of a drug product, in the strictest sense, is defined as "complete absence of microorganisms in the product." However, the test for sterility as described in the Pharmacopoeias [I], is not a test on which any strong reliance can be based tor sterility assurance of the product. Due to limitations in testing [2]. the test for sterility is not a suitable release test to verify that a pr...

The effect of releases of bisexual (males and female) and unisexual (male only) sterilized medflies was compared in three large field evaluations over a 3-yr period (1995-1997) in southwestern Guatemala. The two strains tested were a genetic sexing strain, Vienna-4/Tol-94, carrying the temperature sensitive tsl gene to eliminate females in the egg stage, and the standard bisexual Petapa strain....