In order to study the hydrolysis, enzymatic or chemical, of the plasma and urinary steroid glucuronides, as well as the biosynthesis, the isolation of the individual 17-oxosteroids is an absolute necessity. Schneider and Lewbart (1959) employed counter-current distribution techniques to isolate several steroid glucuronides. Androsterone glucuronide was isolated by Edwards and Kellie (1956) and ...

The behaviour of various C(19) and C(18) steroids as substrates for crystalline preparations of cortisone reductase (EC 1.1.1.53) is described. 3alpha(Axial,3R)-, 3alpha(equatorial,3R)- and 3beta(axial,3S)-hydroxy steroid-NAD oxidoreductase activities are demonstrated. Four pairs of the substrates differed only in the shape of the a/b ring junction, three pairs differed only in substitution at ...

1. If two compounds are substrates for a single enzyme, and do not form any ternary complex with the enzyme or combine directly with each other, then the total initial rate of reaction for a mixture of the two compounds may be greater than the rate for either compound alone, or may lie between the rates for the compounds alone. It is the concentration of the compound with the higher maximum vel...

The objective of this study was to regulate the reproduction and levels of gonadal steroids in 3-year-old protandrous black porgy (Acanthopagrus schlegeli) by treatment with microencapsulated D-Trp6-luteinizing hormone-releasing hormone (LH-RH analog) during the prespawning season. Twenty-four previously male black porgy were equally divided into 2 groups and injected with vehicle (control grou...

It has recently been shown in our laboratories that a soil microorganism, Pseudomonas No. 7, can grow in pure culture in the presence of testosterone as the sole source of carbon (1). Manometrically, oxygen uptake and carbon dioxide evolut,ion were noted with this st,eroid as substrate. At the end of the incubation period, definite amounts of testosterone had disappeared. These results were int...

The expression of the two gene clusters encoding the particulate methane monooxygenase (pMMO) in Methylococcus capsulatus Bath was assessed by analysis of transcripts and by use of chromosomal gene fusions. The results suggest that the two clusters are functionally redundant but that relative expression alters depending on the copper levels available for growth.

An infertile male patient with a 45,X peripheral blood karyotype and a 45,X/46,X,del(Y)(pter----q11.1:) mosaic skin fibroblast karyotype is described. Steroid sulphatase (STS) activity was normal. Recombinant DNA studies using Y chromosome specific probes suggest that almost the entire long arm of the Y chromosome is deleted.

The sex steroid-binding protein of human plasma SBP (or sex hormone-binding globulin, SHBG) was specifically inhibited with the alkylating affinity label, 17 beta-[[( 2-14C]bromoacetyl)oxy]-5 alpha-androstan-3-one. The natural ligand, 5 alpha-dihydrotestosterone, was shown to protect against inactivation and labeling. The steroid-binding activity of the protein was abolished when approximately ...

Cushing's syndrome (CS) is common after oral steroid use and has also been reported following topical or inhaled use, but it is extremely uncommon after intranasal administration. In this paper, we present the case of a child who developed CS after intranasal application of combined moxifloxacin-dexamethasone eye drops for epistaxis for a period of 3 months. CS caused by ocular preparations of ...

A carbonaceous (heterotrophic) activated sludge process (ASP), nitrifying ASP and a nitrifying/denitrifying ASP have been studied to examine the role of process type in steroid estrogen removal. Biodegradation efficiencies for total steroid estrogens (Sigma(EST)) of 80 and 91% were recorded for the nitrifying/denitrifying ASP and nitrifying ASP respectively. Total estrogen biodegradation (Sigma...