OBJECTIVE To compare the results of Clostridium Difficile (CD) on culture with detection of C. difficile toxin by Enzyme Immunoassay (EIA) in the stool specimens of hospitalized patients with antibiotic associated diarrhoea (AAD). PATIENTS AND METHODS The study included 80 adult patients with AAD and 20 adult patients with non-AAD. Stool specimens of all these subjects were inoculated on cycl...

Clostridium difficile is a major cause of nosocomial antibiotic-associated infectious diarrhea and pseudomembranous colitis. Detection of C. difficile by anaerobic bacterial culture and/or cytotoxicity assays has been largely replaced by rapid enzyme immunoassays (EIA). However, due to the lack of sensitivity of stool EIA, we developed a multiplex real-time PCR assay targeting the C. difficile ...

A total of 132 fecal specimens containing verotoxin (VT) were subjected to counter-current immunoelectrophoresis (CIE). Of these, 113 (85.6%) were found to be positive by CIE. Another 71 stool specimens containing E. coli serogroup O157 but with flagellar antigens other than H7 were tested for verotoxin by CIE. These stool specimens were negative for VT on Vero cell monolayers. Of these 71 stoo...

BACKGROUND There is a significant demand for colorectal cancer (CRC) screening methods that are noninvasive, inexpensive, and capable of accurately detecting early stage tumors. It has been shown that models based on the gut microbiota can complement the fecal occult blood test and fecal immunochemical test (FIT). However, a barrier to microbiota-based screening is the need to collect and store...

Liquid-based microbiology (LBM) devices incorporating flocked swabs and preservation medium ease transport of specimens and improve specimen yield compared to traditional fiber wound swabs; however, the performance of LBM collection devices has not been evaluated in many molecular assays. It is unclear how the differences in matrix and specimen loading with an LBM device will affect test perfor...

BACKGROUND stool type represents an important semiologic part of medical interviews. The Bristol Scale Stool Form is a clinical tool to evaluate stool consistency and form. The aim of this study was to translate and adapt the Bristol Scale Stool Form into Spanish. Differences in validation results between health professionals and patients surveyed were also evaluated. METHODS the study popula...

AIM To compare (1) demographics in urea breath test (UBT) vs endoscopy patients; and (2) the molecular detection of antibiotic resistance in stool vs biopsy samples. METHODS Six hundred and sixteen adult patients undergoing endoscopy or a UBT were prospectively recruited to the study. The GenoType HelicoDR assay was used to detect Helicobacter pylori (H. pylori) and antibiotic resistance usin...

BACKGROUND In Schistosoma mansoni infection, diagnosis and control after treatment mainly rely on parasitological stool investigations which are laborious and have limited sensitivity. PCR methods have shown equal or superior sensitivity but preservation and storage methods limit their use in the field. Therefore, the use of occult blood detection cards (fecal cards) for easy sampling and stora...

introduction: helicobacter pylori infection has various clinical features. one of the most common presentations of this infection is upper abdominal pain. complications such as gastritis, gastric ulcer, gastric carcinoma and malt lymphoma mandate early diagnosis of h.p infection by a low cost and non invasive manner. the aim of this study was to evaluate the diagnostic role of h.p stool antigen...

The aim of current studies was to validate the direct plating of a stool sample for Listeria monocytogenes detection, using selective medium Palcam agar with Palcam selective supplement. Validation was performed using stool samples collected from healthy humans inoculated with Listeria sp. strains. Stool samples were frozen to determine the influence of freezing on method robustness. The presen...