PURPOSE In the pediatric population, several different antibiotic regimens are currently recommended for the treatment of otitis media. This study investigated whether therapy for otitis media was associated with the emergence of antibiotic-resistant oral bacteria. METHODS Streptococcus sanguis (S. sanguis) was isolated from supragingival dental plaque of children after a recent course of ant...

Gingivitis is an inflammatory destructive disease mainly caused by microbial plaques. Gram-positive and gram-negative bacilli or cocci have been isolated from dental plaque and gingivitis. This study was going to isolate and characterize the pathogenic bacteria from gingival of 100 patients with oral gingivitis. Enriched and specific media used for isolation of bacteria. Identification of isola...

Biofilms are polymicrobial, with diverse bacterial species competing for limited space and nutrients. Under healthy conditions, the different species in biofilms maintain an ecological balance. This balance can be disturbed by environmental factors and interspecies interactions. These perturbations can enable dominant growth of certain species, leading to disease. To model clinically relevant i...

Chromosomal DNA from Streptococcus sanguis FW213 was partially digested with EcoRI and ligated into the positive-selection cloning vector pOP203(A2+). The ligation mixture was used to transform Escherichia coli K-12, and 4,500 transformants were examined. The tetracycline-resistant colonies had inserts averaging 3.2 kilobases. The entire colony bank was screened by colony immunoassay with polyc...

gingivitis is an inflammatory destructive disease mainly caused by microbial plaques. gram-positive and gram-negative bacilli or cocci have been isolated from dental plaque and gingivitis. this study was going to isolate and characterize the pathogenic bacteria from gingival of 100 patients with oral gingivitis. enriched and specific media used for isolation of bacteria. identification of isola...