Background: Colorectal cancer (CRC) is one of the deadliest cancers worldwide and long-term survival not guaranteed in metastatic disease despite current multidisciplinary therapies. A new compound 2,3,5,4′-Tetrahydroxystilbene (TG1), derived from THSG (2,3,5,4′-Tetrahydroxystilbene-2-O-β-D-Glucoside), has been developed, its anticancer ability against CRC verified this study. Methods: HCT116, ...

The toxicity of individual disinfectants has been studied in vitro using human cell cultures (HT-29 (epithelial-like cells colon adenocarcinoma), HEK 293 (human embryonic kidney cells)) to create a model for assessing the residual amounts that can enter milk person. Standard tests have used assess viability and amount: methyl tetrazolium (MTT) test, neutral red staining (NRP), sulforhodamine B ...

Cell cycle arrest is closely linked to apoptosis. Isomorellin-a caged xanthone isolated from Garcinia hanburyi-induced apoptosis in cholangiocarcinoma (CCA) cell lines. To elucidate potential anticancer mechanisms, we investigated the effects of isomorellin on the growth, cell cycle progression, cell cycle regulated protein expression and nuclear factor-kappa B (NF-κB) activation of KKU-100 and...

Cephalotaxus mannii Hook.f is a rare medicinal plant used for the treatment of leukemia, however, its fungal endophytes with antibacterial and anticancer properties have not been exploited yet. In this study, six isolates were recovered from roots C. collected in Ha Giang province, Vietnam. Among them, ethyl acetate crude extract strain WQF5 exhibited strong activity against 5 tested pathogens ...

Betulin is a lupane-type pentacyclic triterpene. It characterized by range of biological properties, including anti-cancer and anti-inflammatory activities. also an origin compound for obtaining derivatives with higher activity better bioavailability. Chronic inflammation stimulates the formation pro-cancer microenvironment, promoting tumor growth, cell migration, neoangiogenesis. Many factors,...

In order to investigate the role of translationally-controlled tumor protein (TCTP) in cholangiocarcinoma (CCA) progression and metastasis, TCTP protein staining in paraffin-embedded sections of human CCA tissue samples was examined using immunohistochemistry, and its expression was subsequently compared with clinicopathological parameters. Small interfering RNA (siRNA) targeting TCTP (siTCTP) ...

Lung cancer is the major cause of cancer death among men. A number of natural compounds have proven to be useful in the treatmet of lung cancer. This study was aimed to determine cytotoxic and apoptotoic effects of a natural compound 3-O-α-L-arabinosyl oleanolic acid (3-O-L-AO) isolated from Schumacheria castaneifolia in non-small-cell lung cancer (NCI-H292) cells. Cytotoxic effects of 3-O-L-AO...

The reliable identification of astrocytes for physiological measurements was always time-consuming and difficult. Recently, the fluorescent dye sulforhodamine 101 (SR101) was reported to label cortical glial cells in vivo [Nimmerjahn A, Kirchhoff F, Kerr JN, Helmchen F. Sulforhodamine 101 as a specific marker of astroglia in the neocortex in vivo. Nat Methods 2004;1:31-7]. We adapted this techn...

The cytotoxic activity and the chemical composition of the dichloromethane/methanol root extract of Linum scabrellum Planchon (Linaceae) were analyzed. Using NMR spectra and mass spectrometry analyses of the extract we identified eight main constituents: oleic acid (1), octadecenoic acid (2), stigmasterol (3), α-amyrin (4), pinoresinol (5), 6 methoxypodophyllotoxin (6), coniferin (7), and 6-met...

Edible yams are tropical crops that serve as important staple foods in many parts of the world. The rhizome of Dioscorea japonica , well-known as "Japanese yam", is a food and medicinal source known as "San Yak" in Korea. Bioassay-guided fractionation and chemical investigation of the extract of this yam resulted in the identification of two new withanolides, named dioscorolide A (1) and diosco...