We have identified two novel periplasmic/cell wall polypeptides that specifically accumulate during sulfur limitation of Chlamydomonas reinhardtii. These polypeptides, present at high levels in the extracellular polypeptide fraction from a sulfur-deprived, cell wall-minus C. reinhardtii strain, have apparent molecular masses of 76 and 88 kD and are designated Ecp76 and Ecp88. N-terminal sequenc...

Biogenesis of iron-sulfur clusters requires a concerted delivery of iron and sulfur to target proteins. It is now clear that sulfur in iron-sulfur clusters is derived from L-cysteine via cysteine desulfurases. However, the specific iron donor for the iron-sulfur cluster assembly still remains elusive. Previous studies showed that IscA, a member of the iron-sulfur cluster assembly machinery in E...

S is a common corrosion medium for austenitic stainless steels. The severe intergranular fracture of steels occurs in sulfur environments. In this paper, the permeation at different atomic positions three symmetric tilt grain boundary types, i.e., Σ5(210), Σ5(310), and Σ9(114) have been computed using first-principles calculations. has strongest segregation tendency Σ5(210) boundary. A high con...

Ni-doped chromite anodes were integrated into electrolyte-supported cells (ESC) with 5×5 cm2 size and investigated in fuel cell mode H2/H2O gas. Both a stoichiometric nominally A-site deficient anode material showed promising performance at 860 °C approaching the ones of state-of-the-art Ni/Gd-doped ceria (CGO) anodes. While difference polarization resistance was small, an increased ohmic perov...

The activity of photosynthesizing sulfur bacteria in a continuous culture was studied. The bacteria were isolated from the natural environment with the use of the Winogradski column. Isolated bacteria were cultured in synthetic medium and in the effluent from the column containig HS-. Sulphides, the main product of reduction of sulfates in phosphogypsum, were used by green sulfur bacteria in th...

A Pseudomonas aeruginosa AK6U strain produced rhamnolipid biosurfactants to variable extents when grown on MgSO4 or organosulfur compounds as sulfur sources and glucose as a carbon source. Organosulfur cultures produced much higher biosurfactants amounts compared to the MgSO4 cultures. The surface tension of the growth medium was reduced from 72 mN/m to 54 and 30 mN/m in cultures containing MgS...

Various bacteria, actinomycetes, and filamentous fungi decomposed methionine, but only certain aerobic bacteria isolated from soil decomposed it in the absence of other organic substrates. These bacteria could grow on methionine as the only organic substrate and source of nitrogen and sulfur. Methionine was first deaminated and then demethiolated with production of methanethiol, part of which w...

Iron-sulfur clusters are prosthetic groups that are required for the function of numerous enzymes in the cell, including enzymes important in respiration, photosynthesis, and nitrogen fixation. Here we report cloning of the human homolog of NifS, a cysteine desulfurase that is proposed to supply the inorganic sulfur in iron-sulfur clusters. In human cells, different forms of NifS that localize ...

Cell-free extracts of Bacillus megaterium form beta-cyanoalanine (beta-CNA)-(14)C from Na(14)CN and l-cysteine, O-acetyl-l-serine or, to a lesser extent, l-serine. However, the presence of cyanide in the growth medium does not increase the capacity of cell extracts to catalyze the formation of beta-CNA from cysteine and cyanide. The formation of beta-CNA is readily detected in extracts of cells...

Alkyl- and arylsulfonates were tested as sole added sources of sulfur for the growth of enrichment cultures under strictly anaerobic denitrifying or fermentative conditions. Cultures that utilized taurine, ethylsulfonate, the dyestuffs orange II and acid red I, tolylsulfonate, 2-(4-sulfophenyl)butyrate (SPB), a dialkyltetralinesulfonate, and 1-(4-sulfophenyl)octane were readily obtained. We cho...