Hypoglycemic effects and the use of kelp in diabetes mellitus (DM) model rats induced by alloxan were investigated. Sixty healthy male rats were used to establish DM models by injecting alloxan intraperitoneally. Kelp powder was added to the general forage for the rats. The levels of fasting blood glucose (FBG) were determined by an automatic blood glucose device. Electrochemiluminescence immun...

Extracellular superoxide dismutase (EC-SOD) is one of three mammalian SOD isozymes. Although there is knowledge of the functional role of EC-SOD in arteries, little is known about the function of EC-SOD in other tissues, including the brain. As a first step toward improving our understanding of EC-SOD in the brain, we studied the localization of EC-SOD in the central nervous system of the adult...

Reactive oxygen/nitrogen species suppress myocardial oxygen consumption. In this study, we determined that endogenous hydrogen peroxide through dismutation of superoxide enhances postischemic myocardial blood perfusion and oxygen consumption. Electron paramagnetic resonance oximetry was applied to monitor in vivo tissue Po2 in mouse heart subjected to regional ischemia reperfusion. Heart rate, ...

The aim of this pilot study was to determine the baseline state of oxidative stress indices in patients with Parkinson's disease (PD). Peripheral blood samples of 15 PD subjects were analyzed and compared with ten age matched healthy controls. Patients with PARK2 mutations were also compared with PD patients without mutations. There was significant increase in malondialdehyde content and supero...

To clarify the effect of superoxide dismutase (SOD) on the formation of hydroxyl radical in a standard reaction mixture containing 15 microM of xanthone, 0.1 M of 5,5-dimethyl-1-pyrroline N-oxide (DMPO), and 45 mM of phosphate buffer (pH 7.4) under UVA irradiation, electron paramagnetic resonance (EPR) measurements were performed. SOD enhanced the formation of hydroxyl radicals. The formation o...

Macromolecular crowding can alter the structure and function of biological macromolecules. We used small-angle scattering to measure the effects of macromolecular crowding on the size of a protein complex, SOD (superoxide dismutase). Crowding was induced using 400 MW PEG (polyethylene glycol),TEG (triethylene glycol), α-MG (methyl-α-glucoside), and TMAO (trimethylamine n-oxide). Parallel small-...

Clinacanthus nutans (Burm.f) (CN) Lindau is widely grown in tropical Asia and is an important herbal medicine in China, Malaysia and Thailand. This study inspects the antioxidant activity and protective effects of Clinacanthus nutans extracts on plasmid DNA integrity in E. coli. The antioxidant activities of Clinacanthus nutans are lower compared with green tea. The superoxide dismutase (SOD) a...

Music reduces emotional stress, relieves anxiety, and is utilized while treating various diseases. The present study explored the impacts of music genres at different decibels on oxidation state in brain tissue serum. We carried out 42 male Wistar Albino rats. rats were randomized (six each cage) as a control group groups exposed to noise, rock music, slow for 21 days 4 hours day. At end experi...

Objective: The long-term sequelae of methotrexate (MTX) remain the major cause of concern for both patients and therapists. Therefore, new approaches to decrease MTX side effects are needed. The study was carried out to evaluate the effects of Iris songarica Schrenk (IS) rhizome extract against MTX-induced hepatic and renal injuries in rats. Materials and...

Study was undertaken to investigate the various oxidative stress markers and biochemical parameters in anxious rat. Rats were subjected for short (21 days) and long term (up to 84 days) social isolation; the rats displayed an increase in anxiety on elevated plus maze and open field test relative to control. Various markers of oxidative stress like lipid peroxidation (LPO), reduced glutathione (...