A further characterization of human extracellular superoxide dismutase is reported. The study was especially aimed at the interaction with substances known to interfere with CuZn superoxide dismutase and other superoxide dismutases. Extracellular superoxide dismutase is efficiently inhibited by cyanide and is about 3 times more sensitive than is human CuZn superoxide dismutase. The sensitivity ...

Oxidant-antioxidant balance is known to regulate growth factors and invasion of tumor cells. Manganese superoxide dismutase (MnSOD), copper zinc SOD (CuZnSOD), and extracellular SOD (ECSOD), the first-line antioxidant defenses, were studied in lung carcinomas by immunohistochemical analysis (n = 139, 56, and 37, respectively) and in 8 lung tumor specimens by Western blot analysis and SOD activi...

Superoxide dismutase has been studied in a wide range of organisms (Fridovich, 1975). Early observations suggested that superoxide dismutases isolated from eukaryotes (McCord & Fridovich, 1969; Misra & Fridovich, 1972; Beauchamp & Fridovich, 1973) showed little diversity in their properties, having mol.wts. near 32000 and containing two copper and two zinc atoms per molecule. The activity of th...

In Streptomyces coelicolor, transcription of the sodF genes, encoding Fe-containing superoxide dismutases, is negatively regulated by nickel. Gel mobility shift assays with sodF1 promoter fragments and cell extracts from the A3(2) strain indicate the presence of a nickel-responsive DNA-binding protein, most likely a transcriptional repressor. The boundary for the Ni-responsive cis-acting region...

During its reduction to water, O2 readily gives rise to dangerously reactive intermediates. This threat is diminished by families of defensive enzymes which include the superoxide dismutases, catalases and peroxidases. Free radical chain reactions are controlled by antioxidants, such as ascorbate and the tocopherols, and oxidative damage, which occurs in spite of these defenses, is largely repa...

Heat shock proteins are induced at normal temperatures by oxidants and during reoxygenation following hypoxia. We now report cyanide-resistant O2 consumption increased 30-50% in rat lungs exposed to heat shock or reoxygenation following hypoxia. The synthesis of Cu,Zn superoxide dismutase, but not Mn superoxide dismutase, was increased in rat lung slices by in vivo hyperthermia (39 degrees C), ...