The photoinduced alignment of ferroelectric liquid crystals (FLCs) onto photochemically stable azo-dye films was studied. The alignment quality of FLC display cells depends mainly on the difference between the FLC surface energy and the aligning substrates surface energy; however, the structure and thickness of FLC layers are also important. The effect of the thickness of photoaligning azodye l...

Recombinant baculoviruses have been extensively used as vectors for abundant expression of a large variety of foreign proteins in insect cell cultures. The appeal of the system lies essentially in easy cloning techniques and virus propagation combined with the eukaryotic post-translational modification machinery of the insect cell. Recently, a novel molecular biology tool was established by the...

Most therapeutic antibodies (Abs) target cell surface proteins on tumor and immune cells. Cloning of Ab gene libraries in E. coli and their display on bacteriophages is commonly used to select novel therapeutic Abs binding target antigens, either purified or expressed on cells. However, the sticky nature of bacteriophages renders phage display selections on cells challenging. We previously repo...

Experiments in Surface Perception using a Fingertip Haptic Display by Steven Craig Venema Chairperson of Supervisory Committee: Professor Blake Hannaford Department of Electrical Engineering A haptic display provides the mechanical analog of a physical environment for our sense of touch in a virtual reality (VR) simulation system. In this dissertation, we explore the design and implementation o...

We have analyzed the surface glycoproteins of resting and in vitro activated human T lymphocytes by the galactose oxidase/NaB3H4 and the periodate/NaB3H4 labeling techniques. The labeled glycoproteins were separated by polyacrylamide slab gel electrophoresis and visualized by fluorography. A "new" glycoprotein with an apparent molecular weight of 130,000 (GP130) was strongly labeled on alloanti...

Expressing proteins of interest as fusions to proteins of the bacterial envelope is a powerful technique with many biotechnological and medical applications. Autotransporters have recently emerged as a good tool for bacterial surface display. These proteins are composed of an N-terminal signal peptide, followed by a passenger domain and a translocator domain that mediates the outer membrane tra...

The gene encoding organophosphorus hydrolase (OPH) from Flavobacterium species was expressed on the cell surface of Saccharomyces cerevisiae MT8-1 using a glycosylphosphatidylinositol (GPI) anchor linked to the C-terminal region of OPH. Immunofluorescence microscopy confirmed the localization of OPH on the cell surface, and fluorescence intensity measurement of cells revealed that 1.4 x 10(4) m...

In this paper, a steerable, interactive projection display that has the shape of a disk is presented. Interactivity is provided through sensitivity to the contact of multiple fingertips and is achieved through the use of a RGBD camera. The surface is mounted on two gimbals which, in turn, provide two rotational degrees of freedom. Modulation of surface posture supports the ergonomy of the devic...

Copyright is held by the author/owner(s). ITS’12, November 11-14, 2012, Cambridge, Massachusetts,USA. Abstract In this research, we proposed a new display with a dynamically changeable softness parameter called ”ClaytricSurface”. While conventional displays generally have fixed softness, we focused on interactive displays with a controllable softness property. We developed a prototype system by...

Projector-based display systems have been used in computer graphics for about as long as the field has existed. While projector-based systems have many advantages, a significant disadvantage is the need to obtain an accurate analytical model of the mechanical setup, including the external parameters of the projectors, and a description of the display surface. We introduce a new method for the l...