Swine influenza was first recognized as a disease entity during the 1918 "Spanish flu" pandemic. The aim of this work was to determine the virulence of a plasmid-derived human 1918 pandemic H1N1 influenza virus (reconstructed 1918, or 1918/rec, virus) in swine using a plasmid-derived A/swine/Iowa/15/1930 H1N1 virus (1930/rec virus), representing the first isolated influenza virus, as a referenc...

BACKGROUND H3N2 is one of the main subtypes of influenza virus which circulates in human and swine population throughout the world. OBJECTIVES To investigate the genetic correlation between H3N2 human and swine influenza viruses from the same region during the same season. STUDY DESIGN Five H3N2 human and four H3N2 swine influenza viruses were isolated from Guangdong province of China in th...

The novel influenza A (H1N1) virus is now rapidly spreading across the world. Early detection is one of the most effective measures to prevent further transmission of the virus. 4 sets of proprietary primers and probes designed for detection of influenza A viruses (InfA), human and swine H1N1 viruses (SH1), the novel H1N1 viruses (NH1) and RNaseP gene (RP) respectively were pooled together in a...

BACKGROUND Influenza A viruses are medically important viral pathogens that cause significant mortality and morbidity throughout the world. The recent emergence of a novel human influenza A virus (H1N1) poses a serious health threat. Molecular tests for rapid detection of this virus are urgently needed. METHODS We developed a conventional 1-step RT-PCR assay and a 1-step quantitative real-tim...

Schaefer R., Zanella J.R.C., Brentano L., Vincent A.L., Ritterbusch G.A., Silveira S., Caron L. & Mores N. 2011. Isolation and characterization of a pandemic H1N1 influenza virus in pigs in Brazil. Pesquisa Veterinária Brasileira 31(9):761-767. Embrapa Swine and Poultry Research Center, BR153, Km110, Vila Tamanduá, Concórdia, SC 89700000, Brazil. E-mail: [email protected] Influenza A viru...

Since the emergence of the 2009 pandemic (H1N1) virus (2009/H1N1) in April 2009, cases of transmission from humans to pigs have been reported frequently. In our previous studies, four 2009/H1N1 variants were isolated from pigs. To better understand the phenotypic differences of the pig isolates compared with the human isolate, in this study mice were inoculated intranasally with different 2009/...

Schaefer R., Zanella J.R.C., Brentano L., Vincent A.L., Ritterbusch G.A., Silveira S., Caron L. & Mores N. 2011. Isolation and characterization of a pandemic H1N1 influenza virus in pigs in Brazil. Pesquisa Veterinária Brasileira 31(9):761-767. Embrapa Swine and Poultry Research Center, BR153, Km110, Vila Tamanduá, Concórdia, SC 89700000, Brazil. E-mail: [email protected] Influenza A viru...

From August 17 to December 23, 2011, CDC received reports of 12 human infections with influenza A (H3N2)v viruses that have the matrix (M) gene from the influenza A (H1N1)pdm09 virus (formerly called swine-origin influenza A [H3N2] and pandemic influenza A [H1N1] 2009 viruses, respectively). The 12 cases occurred in five states (Indiana, Iowa, Maine, Pennsylvania, and West Virginia), and 11 wer...

INTRODUCTION We investigated the frequency of H274Y-positive swine-origin 2009 A (H1N1) influenza virus outbreak in Thailand during May-August 2009.  METHODOLOGY This study sought to find Oseltamivir resistance mutation H274Y by using pyrosequencing. RESULTS From 8,710 real-time RT-PCR swine-origin 2009 A(H1N1) influenza virus-positive specimens, 100 randomly selected samples identified one...

The Centers for Disease Control (CDC) predicted a resurgence of Swine-origin Influenza A (novel 2009 H1N1) pneumonia, hospitalizations and deaths during the 2009-2010 flu season. Immunocompromised patients are at higher risk to contract it and may present (atypically) with greater morbidity and mortality. We report the first radiographic description of CDC-confirmed swine-origin influenza A (no...