background: breast cancer is the most common cancer in women. non-coding rnasespecially mirnas have important regulatory roles in cancer. mirnas are 21-24 nucleotides which have different levels of expression between tumors and normal tissues. in this study, we have analyzed expression level of mir-520d in three different groups of breast cancer. methods: fifty nine samples were divided into di...

Background: Stephanofilaria spp. nematodes are associated with cutaneous lesions in cattle and other livestock mammalian wildlife species. In Australia, Haematobia irritans exigua, commonly known as buffalo fly (BF) transmits a well-described but presently unnamed species of Stephanofilaria, which has been speculatively implicated the aetiology BF lesions. The sensitivity current techniques for...

A library of enriched marine natural product fractions was screened for their antiplasmodial activity using a SYBR green I fluorescence-based assay. Fractions derived from a sponge of the genus Spongosorites exhibited potent inhibition of Plasmodium falciparum growth. This genus of sponge has been reported to contain the nortopsentin and topsentin class of bis-indole imidazole alkaloids. This i...

PE Biosystems has developed guidelines enabling streamlined design and implementation of real-time quantitative PCR assays. The use of these guidelines makes it easy to apply either the fluorogenic 5 ́ nuclease assay or SYBR Green I double-stranded DNA binding dye chemistry to any real-time quantitative PCR system. Specific assay design and optimization guidelines minimize the time and cost of a...

In this study, we developed a SYBR Green I real-time PCR method for the rapid and sensitive detection of novel porcine parvovirus 7 (PPV7). Specific primers were designed based on highly conserved region within Capsid gene PPV7. The established was 1,000 times more than conventional had limit 35.6 copies. This specific no cross-reactions with PCV2, PCV3, PRV, PEDV, PPV1, PPV6. Experiments testi...

Abstract: We optimized a methodology for plankton environmental DNA detection of the invasive golden mussel and validated it in samples from Southern Brazil reservoir. Limnoperna fortunei is successful alien species that causes significant impacts on freshwater ecosystems. adjusted to detect L. samples, with SYBR Green assay. Based standard curve analysis, observed theoretical minimal qPCR leve...