A sensitive method using the Technicon Amino Acid Autoanalyzer was developed for the determination of l-tyrosine O-sulphate in mammalian urine. Normal rabbit urine was shown to contain a material that has properties identical with l-tyrosine O-sulphate. The material is also a normal constituent of rat, sheep, calf and mouse urine and is excreted by these animals in quantities ranging from 0.1 t...

An AutoAnalyzer method has been developed in which the recently introduced reagent for iron, 3. (2.pyridyl).5,6.bis(4.phenylsulfonic acid).1,2,4.triazine (“ferrozine”), is used. With the Technicon colorimeter or Gilford 300-N spectrophotometer in use, sampling rates may be 30/h or 50/h, respectively. Sample volume is 0.8 ml, and measurements are made at 560 nm. A procedure for measuring total i...

A procedure is described for the simultaneous, automatic determination of serum calcium and magnesium with the AutoAnalyzer, and a dualchannel, double-beam atomic absorption spectrophotometer, the IL Model 153. Advantages over existing procedures include a high rate of analysis (90 samples or 180tests per hour) and low sample consumption (a total of 30 l for both determinations). We have compar...

Ittrich’s method for the determination of total estrogens in urine (1) was adapted for automation on the AutoAnalyzer, using a continuous digester module for the combined operation of phase exchange and color development. The automated method gave good recovery and reproducibility, and correlated well with the manual method. It increased the capacity of this laboratory for estrogen determinatio...