The ascomycete Trichoderma reesei is a paradigm for the regulation and production of plant cell wall-degrading enzymes, including xylanases. Four xylanases, including XYN1 and XYN2 of glycosyl hydrolase family 11 (GH11), the GH10 XYN3, and the GH30 XYN4, were already described. By genome mining, we identified a fifth xylanase, XYN5, belonging to GH11. Transcriptional analysis reveals that the e...

A bacterial xylanase gene, Nonomuraea flexuosa xyn11A, was expressed in the filamentous fungus Trichoderma reesei from the strong cellobiohydrolase 1 promoter as fusions to a variety of carrier polypeptides. By using single-copy isogenic transformants, it was shown that production of this xylanase was clearly increased (up to 820 mg/liter) when it was produced as a fusion protein with a carrier...

The conidium plays a critical role in the life cycle of many filamentous fungi, being the primary means for survival under unfavorable conditions. To investigate the transcriptional changes taking place during the transition from growing hyphae to conidia in Trichoderma reesei, microarray experiments were performed. A total of 900 distinct genes were classified as differentially expressed, rela...

BACKGROUND The filamentous fungus Trichoderma reesei has tremendous capability to secrete over 100 g/L of proteins and therefore it would make an excellent host system for production of high levels of therapeutic proteins at low cost. We have developed T. reesei strains suitable for production of therapeutic proteins by reducing the secreted protease activity. Protease activity has been the maj...

Members of genus Trichoderma are known worldwide for mycoparasitism. To gain a better insight into the organization and evolution of their genomes, we used an in silico approach to compare the occurrence, relative abundance and density of SSRs in Trichoderma atroviride, T. harzianum, T. reesei, and T. virens. Our analysis revealed that in all the four genome sequences studied, the occurrence, r...

BACKGROUND ACEII transcription factor plays a significant role in regulating the expression of cellulase and hemicellulase encoding genes. Apart from ACEII, transcription factors such as XYR1, CRE1, HAP2/3/5 complex and ACEI function in a coordinated pattern for regulating the gene expression of cellulases and hemicellulases. Studies have demonstrated that ACEII gene deletion results in decreas...

Trichoderma reesei is a high-yield producer of cellulase for applications in lignocellulosic biomass conversion, but its production requires induction. A mixture glucose and β-disaccharide has been demonstrated to achieve high-level production. However, as inducers, β-disaccharides are prone be hydrolyzed by β-glucosidase (BGL) during fermentation, therefore need supplemented through feeding ov...

This study investigates the potential of golden shower foliage (GSF) fermentable sugars for sustainable bioethanol production. Utilizing chemical and biological pretreatments involving agents such as sodium hydroxide (NaOH), calcium oxide (CaO), Trichoderma reesei. The research analyzed various aspects including reducing sugar coefficient, total sugar, degree polymerization (DP), derived energy...

GOCHEV, V. K. and A. I. KRASTANOV, 2007. Isolation of laccase producing Trichoderma spp. Bulg. J. Agric. Sci., 13: 171-176 Based on its dye decolorization activities four Trichoderma strains were screened as laccase producers. The strains were identified as T. atroviride, T. longibrachiatum, T. reesei, and T. viride. It was determined that it was expedient to screen laccase producing Trichoderm...