Bacteriophage T7 is unable to productively infect Escherichia coli strains carrying the sex factor F. T7 phage development, in terms of RNA and protein synthesis, was compared in T7-infected isogenic F- and F+ strains of E. coli. Slightly less T7 early mRNA and early protein were synthesized in F+ cells. In addition to the defect in T7 late protein production in F+ cells reported by others, sig...

T7 early mRNA produced by a gene 1 amber mutant phage, T7 am27, is chemically stable interms of acid insolubility and T7 DNA hybridizability. However, the messenger activity of individual T7 early mRNA species, transcripts of gene 1, gene 0.7, and gene 1.3, decay with a half-life of about 6.5 min at 30 C. An extensive secondary structure is present in all T7 early mRNA species and is probably r...

The entire T7 bacteriophage genome contains 39937 base pairs (Database NCBI RefSeq N1001604). Here, electrostatic potential distribution around double helical T7 DNA was calculated by Coulomb method using the computer program of Sorokin A.A. ([email protected]). Electrostatic profiles of 17 promoters recognized by T7 phage-specific RNA polymerase were analyzed. It was shown that electrostatic p...

DEVELOPMENT OF RUTHENIUM/TERPYRIDINE COMPLEXES FORWATER OXIDATIONbyDAKSHIKA C. WANNIARACHCHIMay 2014Advisor: Prof. Cláudio VeraniMajor: Chemistry (Physical)Degree: Doctor of PhilosophyThe work presented in the dissertation is focused on developing catalysts forwater oxidation. In this regard, a series of unsysmmetrical ruthenium complexes of type[Ru(t...

This paper brings forward a Digital Earth-based DEM server model – TON (Terrain On the INTERNET). TON takes client/server architecture and closely integrates with WWW server. The server side of TON builds multiresolution terrain database in a quadtree structure, which offers an effective method to retrieve terrain data. The client side of TON integrates with WWW browser as a component, which su...

We update a previously presented Linear Programming (LP) methodology for estimating state level costs for reducing CO2 emissions from existing coal-fired power plants by cofiring switchgrass, a biomass energy crop, and coal. This paper presents national level results of applying the methodology to the entire portion of the United States in which switchgrass could be grown without irrigation. We...

Citrobacter freundii causes opportunistic infections in humans and animals, which are becoming difficult to treat due to increased antibiotic resistance. The aim of this study was to explore phages as potential antimicrobial agents against this opportunistic pathogen. We isolated and characterized five new virulent phages, SH1, SH2, SH3, SH4, and SH5 from sewage samples in Tunisia. Morphologica...

Pondasi tiang pancang merupakan struktur bagian bawah yang menumpu pada tanah keras atau tumpuan padat dan bekerja sebagai penopang berada diatasnya, jika bawahnya kokoh maka atas akan tetap berdiri kokoh, sebaliknya tidak kuat menahan atasnya runtuh, Penelitian ini dilakukan untuk mengetahui daya dukung pondasi tunggal kelompok dengan menggunakan metode Mayerhoff 1956 Luciano Decourt 1996 berd...

Molecular information theory was used to create sequence logos and promoter models for eight phages of the T7 group: T7, phiA1122, T3, phiYeO3-12, SP6, K1-5, gh-1 and K11. When these models were used to scan the corresponding genomes, a significant gap in the individual information distribution was observed between functional promoter sites and other sequences, suggesting that the models can be...