Wnt signaling activates gene expression through the induced formation of complexes between DNA-binding T-cell factors (TCFs) and the transcriptional coactivator beta-catenin. In colorectal cancer, activating Wnt pathway mutations transform epithelial cells through the inappropriate activation of a TCF7L2/TCF4 target gene program. Through a DNA array-based genome-wide analysis of TCF4 chromatin ...

background: niche cells, regulating spermatogonial stem cells (sscs) fate are believed to have a reciprocal communication with sscs. the present study was conducted to evaluate the effect of ssc elimination on the gene expression of glial cell line-derived neurotrophic factor (gdnf), fibroblast growth factor 2 (fgf2) and kit ligand (kitlg), which are the main growth factors regulating sscs deve...

background: peroxisome proliferator activated receptor gamma (pparγ), a member of nuclear receptor superfamily, comprises two isoforms in mouse. these two isoforms are encoded by different mrnas, which are arisen by alternative promoter usage. there are two promoter regions upstream of pparγ gene. a 3 kb fragment, containing several transcription factor binding sites, acts as pparγ1 promoter re...

calorie restriction through ingesting no or minimal amounts of food and caloric beverages for periods of time is called fasting. fasting can affect body through changing in physical and metabolic adaptations, as well as mineral and hormonal status. however, psychological effects and sometimes medical complications are likely in case of inappropriate fasting. fasting is associated with changes i...

background: vast variety of intermediate factors including cell cycle regulators, growth factors, transcription factors, and signaling pathways are involved in hematopoietic stem cell (hsc) commitment and differentiation into distinct lineages. vhl, ecad, and runx3 are among these. epigenetics is currently introduced as a potential mechanism to control the gene regulation. the aim of this study...

background:class ii transactivator (ciita) is a dominanttranscriptional element, controlling numerous genes in theimmune system. ciita is expressed in a constitutive pattern inantigen presenting cells although its expression can occur inother cell types. since the revelation of ciita, there have beenconsiderable advances toward understanding its role as anactivator of mhc ii genes in humans and...

Constitutive activation of Wnt/β-catenin inhibits oligodendrocyte myelination. Tcf7l2/Tcf4, a β-catenin transcriptional partner, is required for oligodendrocyte differentiation. How Tcf7l2 modifies β-catenin signalling and controls myelination remains elusive. Here we define a stage-specific Tcf7l2-regulated transcriptional circuitry in initiating and sustaining oligodendrocyte differentiation....

lentinan a polysaccharide from medicinal mushroom i.e lentinus, has been known to have anticancer properties. telomerase activity is not observed in normal healthy cells, whereas in cancerous cells telomerase expression is high. telomerase represents a promising cancer therapeutic target. we investigated the inhibitory effect of lentinan on telomerase reverse transcriptase gene (htert) which is...

Genome-wide association studies (GWAS) have repeatedly shown an association between non-coding variants in the TCF7L2 locus and risk for type 2 diabetes (T2D), implicating a role for cis-regulatory variation within this locus in disease etiology. Supporting this hypothesis, we previously localized complex regulatory activity to the TCF7L2 T2D-associated interval using an in vivo bacterial artif...

OBJECTIVE The purpose of this study was to determine whether a variation in the transcription factor 7-like 2 (TCF7L2) gene, which influences diabetes risk, is associated with incidence of cancers. RESEARCH DESIGN AND METHODS We related diabetes and TCF7L2 variation with occurrence of several common cancers in a prospective cohort study of 13,117 middle-aged adults initially free of cancer in...