Egg white lysozyme was demonstrated to have antibacterial activity against organisms of concern in food safety, including Listeria monocytogenes and certain strains of Clostridium botulinum. We also found that the food spoilage thermophile Clostridium thermosaccharolyticum was highly susceptible to lysozyme and confirmed that the spoilage organisms Bacillus stearothermophilus and Clostridium ty...

Lactic acid has been identified as one of the top 30 potential building-block chemicals from biomass. Therefore, the search for cheap raw materials is an objective to reduce the production costs. Efficient polymer-grade L-lactic acid production was achieved in this report by a thermophilic strain Bacillus sp. XZL4 using corn stover hydrolyzate as sole carbon source. High L-lactic acid concentra...

The collection of 621 strains of aerobic mesophilic sporeforming bacteria studied by Smith, Gordon, and Clark (1946) included 8 strains that grew slowly, if at all, at 28 C and required a higher temperature than the others for maximum growth. They were closely related and as a group were distinguishable in several respects from the other species. Pending the investigation of more strains the 8 ...

Successful methods to control the damaging weed mold Chaetomium olivaceum (olive green mold) in mushroom beds are not known. An effective antibiotic (named chaetomacin) against C. olivaceum was isolated from a thermophilic Bacillus sp. This compound was shown to be an extremely potent and stable antibiotic, effective over a wide range of both pH (2 to 10) and temperature (-15 to 150 degrees C)....

We studied the segregational stability of plasmids based on pTB913, a 4.5-kb rolling-circle plasmid derived from the thermophilic Bacillus plasmid pTB19. In Bacillus stearothermophilus the stability of pTB913 derivatives appeared to be strain-dependent. In strain CU21 large amounts of single-stranded pTB913 DNA were found and the plasmid was highly unstable at 57 degrees C. In strain NUB3621, h...

Production and hosting by Abstract The Rehai Geothermal Field, located in Tengchong County, in central-western Yunnan Province, is the largest and most intensively studied geothermal field in China. Awide physicochemical diversity of springs (ambient to w97 C; pH from 1.8 to 9.3) provides a multitude of niches for extremophilic microorganisms. A variety of studies have focused on the cultivatio...

A new procedure for production and purification of the thermophilic bacteriophage TP-84 in high yields is described. Cultures of Bacillus stearothermophilus strain 10, enriched with nutrients to obtain heavy growth and to prevent sporulation and maintained at a pH of 6.5, were infected with the phage in a 100-liter fermentor. Addition of magnesium chloride (0.01 M) and a temperature of 58-C we...

Proteins are synthesized and enzymes can function at surprisingly high temperatures in thermophilic micro-organisms. This temperature is about 90°C in the case of Bacillus thermus-aquaticus (A.T.C.C. 25104). Lactate dehydrogenase (EC 1.1.1.27) was isolated from this micro-organism, and the temperature dependence of the rate of pyruvate reduction and thermodynamic parameters of heat inactivation...

The goal of this research was to isolate and identify the thermostable alkaline protease producing bacteria among several native Iranian microorganisms. At the end of screening program, a Bacillus subtilis BP-36 strain producing thermophilic alkaline protease was isolated from a hot spring in Ardebil province. The target enzyme was purified using a one-step Aqueous two-phase systems (ATPS) prot...

thermophilic bacillus sp. gus1, isolated from  a soil  sample obtained from citrus garden, produced at least three proteases as detected by sodium dodecyl sulfate polyacrylamide gel electrophoresis (sds-page) and zymogram analysis. the enzymes were stable in the alkaline ph range (8.0-12.0), with the optimum temperature and ph range of the proteases being 70ºc and 6.0-12.0, respectively. all th...