The thermostability of tyrosinase from three wild type strains of Neurospora crassa has been investigated. For this purpose a sequence comparison of two thermostable and one thermolabile tyrosinase isoenzyme was carried out. It revealed that at position 201 the thermostable enzyme forms share an aspartate residue in contrast to an asparagine residue in the thermolabile form. In addition, one of...

This paper describes a single site, open-label Phase I clinical trial evaluating the safety, tolerability and immunogenicity in healthy volunteers of a herpes simplex polynucleotide vaccine that has previously been shown to enhance immunogenicity and protect against lethal herpes simplex virus type 2 (HSV-2) challenge in mice. Five escalating doses of the vaccine, COR-1, were given by intraderm...

In this paper, some evidence, exper imental or clinical, is presented on the fol lowing subjects : ( I ) The infectious power of a living, sensitized vaccine; (2 ) the absence of immuni ty in vaccinated rabbits to direct gall bladder infect ions; (3 ) the relative pathogenicity, virulence, and toxic i ty of the strain used in the A r m y vaccine; (4 ) local vaccine reactions a f te r typhoid fe...

Historically, most vaccines have been based on killed or live-attenuated infectious agents. Although very successful at immunizing populations against disease, both approaches raise safety concerns and often have limited production capacity. This has resulted in increased emphasis on the development of subunit vaccines. Several recombinant systems have been considered for subunit vaccine manufa...

The advent of thermostable enzymes has led to great advances in molecular biology, such as the development of PCR and ligase chain reaction. However, isolation of naturally thermostable enzymes has been restricted to those existing in thermophylic bacteria. Here, we show that the disaccharide trehalose enables enzymes to maintain their normal activity (thermostabilization) or even to increase a...

Protein engineering approach enables effective immobilisation of thermostable affinity proteins onto polystyrene materials.

Cellular components of thermophilic organisms (enzymes, proteins and nucleic acids) are also thermostable. Apart from high temperature they are also known to withstand denaturants of extremely acidic and alkaline conditions. Thermostable enzymes are highly specific and thus have considerable potential for many industrial applications. The use of such enzymes in maximising reactions accomplished...

A study was conducted to investigate the effects of thermostable phytase supplementation on the growth performance and nutrient digestibility of broilers for 42 d. 1-day-old Arbor Acres chicks with similar initial body weight were randomly divided into 3 treatments consisting 6 pens of 20 chicks per pen. The control group was given maize-soybean meal basal diet and the other two groups were fed...