By using a genetically modified thermostable protein (KTQ5C), we have synthesized protein-stabilized goldnanoclusters (AuNC@KTQ5C) with advantageous properties, such as heat stable fluorescent emission and resistant peroxidase-like activity.

Enzymes are bio-active compounds or catalyst that regulates many chemical reactions in living tissues and cells (Prathyusha and Suneetha, 2011). These enzymes have useful applications in paper, fruit and textile industries. Almost 75% of the estimated sale value among industrial enzymes in 1995 has been contributed by pectinases. As a result, Pectinase became one of the futuristic useful enzyme...

Pectin, an integral component of the plant cell wall, is a recalcitrant substrate against enzymatic challenges by most animals. In characterizing the source of a leaf beetle's (Cassida rubiginosa) pectin-degrading phenotype, we demonstrate its dependency on an extracellular bacterium housed in specialized organs connected to the foregut. Despite possessing the smallest genome (0.27 Mb) of any o...

Variability in growth rate was observed among S strain isolates of Aspergillus flavus from western Arizona. Isolates were grouped into fast and slow growing biotypes and compared for radial growth, and sclerotia, spore, aflatoxin, and pectinase production. Slow and fast biotypes were distributed equally among three collection areas with 35% of 300 S strain isolates classified as the slow biotyp...

Thermostable enzymes have applications in biosensors and bioreactors. Overexpression of thermostable enzyme-encoding genes from thermophiles Escherichia coli is necessary to produce sufficient quantities such enzymes. Deinococcus gerthermalis a moderately thermophilic bacterium has Nicotinamide adenine dinucleotide (NADH) an important coenzyme for various dehydrogenases. NADH oxidase (Nox) cata...

The adenosine A2A receptor (A2AR) belongs to the superfamily of membrane proteins called the G-protein-coupled receptors (GPCRs) that form one of the largest superfamilies of drug targets. Deriving thermostable mutants has been one of the strategies used for crystallization of A2AR in both the agonist and antagonist bound conformational states. The crystal structures do not reveal differences i...

BACKGROUND The concerted action of three complementary cellulases from Clostridium thermocellum, engineered to be stable at elevated temperatures, was examined on a cellulosic substrate and compared to that of the wild-type enzymes. Exoglucanase Cel48S and endoglucanase Cel8A, both key elements of the natural cellulosome from this bacterium, were engineered previously for increased thermostabil...

Purification and biochemical characterization of pectinase produced by Aspergillus fumigatus isolated from soil decomposing plant materialsRaphael Emuebie Okonji, Babamotemi Oluwasola Itakorode, Julius Ovie Ovumedia, Odunayo Selimot Adedeji

Macerase, a pectinase-containing enzyme mixture, was used to digest cell walls isolated from cultured pear cells. Following digestion, the reaction mixture was boiled to inactivate enzymes. Addition of soluble aliquots of the mixture to suspension cultures of pear cells led to a rapid and transient production of ethylene by the cells.

In this study, microbial utilization of coffee pulp waste to produce three carbohydrases (cellulase, pectinase, and xylanase) has been investigated. An isolate Listeria sp. ISH-16 which grown in liquid medium containing 0.5% alkali extract can grow well with density reach 107 x 58.5 cell/mL. Further, the culture optimum produced pectinase after 72 hours incubation at 37oC, but for cellulase xyl...