ii Chapter I General Introduction 1 Chapter II Literature review 4 2.1 Aristolochia 5 2.1.1 Aristolochia consimilis 5 2.2 Pharmacology 6 2.3 Aristolochic Acid 8 2.3.1 Toxicity 9 2.4 Methods for analyzing aristolochic acids 11 2.4.1 Extraction of aristolochic acids 11 2.4.2 Thin layer chromatography (TLC) 12 2.4.3 High performance liquid chromatography (HPLC) 12 2.5 Objective 14 2.6 Approach 14 ...

To study physico-chemical, phytochemical and high performance thin layer chromatography of a Siddha drug “Kadukkai Legiyam” (KL). The prepared Kadukkai Legiyam (KL) was as per the standard operating procedures mentioned in literature. Then subjected to physicochemical parameters, screening, chromatographic photo documentation (TLC), (HPTLC) finger print profile hexane, chloroform, ethanol hydro...

Adsorption thin layer chromatography (NP-TLC) with densitometry has been established for the identification and the quantification of acetaminophen in three leading commercial products of pharmaceutical tablets coded as brand: P1 (Product no. 1), P2 (Product no. 2), and P3 (Product no. 3). Applied chromatographic conditions have separated acetaminophen from its related substances, namely, 4-ami...

A high performance thin layer chromatography system (HPTLC) for the separation of vitamin B2 and B12 has been developed. The separation was successfully using a solvent system of methanol, water, ammonia 7.3.1 (V/V) as mobile phase on HPTLC plates impregnated with boric acid. The effect of other mobile phases on the separation of vitamins was also examined. The method is based on different beha...

Kaempferia parviflora Wall. ex Baker is a medicinal plant found in the upper Northeastern regions of Thailand, which belongs to Zingiberaceae family. The present study aims to investigate the standardization parameters, to analyze chemical constituents of volatile oil by gas chromatography-mass spectrometry, and to determine the content of 5,7-dimethoxyflavone in K. parviflora rhizomes by thin-...

Simple, cheap and green protocol for effective conversion of alcohols to corresponding carbonyl compounds in the presence of H14[NaP5W30O110] and ammonium nitrate is reported. The products were characterized by FT-IR and comparison of their physical properties with those reported in the literatures. The progress of the reaction was monitored by thin layer chromatography (TLC) technique. The ali...

Different solvent extracts of a red algae, Hypnea flagelliformis,and two brown algae, Cystoseira myrica and Sargassum boveanum, collected from the Persian Gulf coast were subjected to different bioassays including: 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging assay, antibacterial and antifungal activity by thin layer chromatography (TLC)-bioautography, agar disc diffusion (ADD) ...

A highly-sensitive analytical method for the detection of p-hydroxymethamphetamine (pOHMA) in urine is presented. The proposed method combines liquid-liquid extraction with acetonitrile and solid-phase extraction by thin-layer chromatography (TLC) with oxidation using potassium hexacyanoferrate(III) and sodium hydroxide to detect the fluorophor of pOHMA. The detection limit for pOHMA is 10 ng (...

A method is described for the fluorometric determination of fecal bile acids. The method combines solvent extraction and thin-layer chromatography (TLC) isolation tech nics with a fluorometric method for measuring total bile acids. Recoveries of individual bile acids added to feces averaged 107% with a standard deviation of ± 5.6%. Bile acid excretion in normal human subjects on a self-selected...

background: the current study was conducted to compare elisa with thin layer chromatography (tlc) methods for diagnosis of morphine in the urine. methods: positive urine samples for morphine confirmed by immunochromatographic strips were collected from the imam reza toxicology laboratory, mashhad university of medical sciences, mashhad, iran in 2012 for the current study. then, the collated uri...