Lack of differentiated functions of the tissue of origin in tissue culture thought to be due to dedifferentiation was shown to be due to selective overgrowth of fibroblasts. Enrichment culture techniques, (alternate animal and culture passage), designed to give the functionally differentiated cells selective advantage over the fibroblasts resulted in a large number of functionally differentiate...

CONTENTS History of Plant Tissue Culture and Biotechnology Scope and Importance of Biotechnology Tissue and Cell Culture Micropropagation Anther Culture Production of Secondary Metabolites Protoplasts Isolation and Fusion Somatic Hybrids Somaclonal Variation Germplasm Conservation Genetic Engineering and Gene Technology Methods for Gene Transfer Generation of Transgenic Plants and their identif...

background & aim: investigating the possibility of using medicinal plant’s seeds substitute for agar gel in plant tissue culture and determining the optimal practical conditions of them is the aim of this study. experimental: in order to study the new gelling agents for using in plant tissue culture, we designed a tow part experiment by means of the seeds of medicinal plants such as linum u...

introduction: this study was designed to investigate the characters of the human endometrial epithelial cells when cultured on either plastic or ecm (extracellular matrix) gel surface and to study ultrastructural features of cultured cells in comparison to endometrial tissue. material and methods: human endometrial tissue was obtained from patients who had undergone hysterectomy for uterine myo...

objective articular cartilage as an avascular skeletal tissue possesses limited capacity to heal. on the other hand, it is believed that the regeneration capacity of each tissue is largely related to its stem cell contents. little is known about the presence of mesenchymal stem cells in articular cartilage tissue. this subject is investigated in the present study. materials and methods articula...

Objective Adult stem cells which are derived from different tissues, with their unique abilities to self-renew and differentiate into various phenotypes have the potential for cell therapy and tissue engineering. Human adipose tissue is an appropriate source of mesenchymal stem cells with wide differentiation potential for tissue engineering research. In this study isolated stem cells from hum...