introduction: organ donation is one of the surviving procedures which can increase the life expectancy of end-stage patients. inappropriate beliefs and attitude of individuals to organ donation, their poor knowledge, and the socio-economic level are one of the most important barriers for organ donation. therefore, here knowledge and attitude levels among relatives of trauma patients regarding o...

Intravital microscopy has proven to be a powerful tool for studying microvascular physiology. In this study, we propose gas exchange system compatible with intravital that can used impose perturbations small localized regions in skeletal muscles or other tissues imaged using conventional inverted microscopes. We demonstrated the effectiveness of by locally manipulating oxygen concentrations rat...

A Mathematical model for the two phase flow of blood in a narrow capillary surrounded by the tissue has been presented in this paper. We have developed the earlier two phase models of blood flow in capillaries into a model of capillary-tissue fluid exchange system by including porous tissue enclosing the capillary. Blood is not a homogeneous fluid, It is a suspension. The suspended blood cells ...

The malignant glioma is the most common primary human brain tumor. Its tendency to invade away from the primary tumor mass is considered a leading cause of tumor recurrence and treatment failure. Accordingly, the molecular pathogenesis of glioma invasion is currently under investigation. Previously, we examined a gene expression array database comparing human gliomas to nonneoplastic controls a...

BACKGROUND Control of the extracellular fluid volume is one of the most indispensable issues for homeostasis of the internal milieu. However, complex interdependence of the pressures involved in determination of fluid exchange makes it difficult to predict a steady-state tissue volume under various physiological conditions without mathematical approaches. METHODS Here, we developed a capillar...

Proteoglycans were extracted from bovine lung gas exchange tissue, pleura, and bronchioles with 4.0 M guanidinium chloride at 5 degrees C in the presence of protease inhibitors. Preliminary purification of the proteoglycans was achieved by an initial CsCl isopycnic centrifugation (rho 0 = 1.33) and through precipitation with cetylpyridinium chloride in 0.5 M KCl. Further purification and fracti...

The developing seed essentially relies on external oxygen to fuel aerobic respiration, but it is currently unknown how oxygen diffuses into and within the seed, which structural pathways are used and what finally limits gas exchange. By applying synchrotron X-ray computed tomography to developing oilseed rape seeds we uncovered void spaces, and analysed their three-dimensional assembly. Both th...

BACKGROUND Extracorporeal membrane oxygenation (ECMO) can replace the lungs' gas exchange capacity in refractory lung failure. However, its limited hemocompatibility, the activation of the coagulation and complement system as well as plasma leakage and protein deposition hamper mid- to long-term use and have constrained the development of an implantable lung assist device. In a tissue engineeri...