Two holding media were compared for their effects on total coliform recovery by the delayed-incubation membrane filter procedure. LES-MF holding medium contains tryptone, m-Endo broth, dipotassium hydrogen phosphate, sodium benzoate, sulfanilamide, para-aminobenzoic acid, and cycloheximide (pH 7.0). m-ST holding medium contains ethanol, sodium monophosphate, dipotassium hydrogen phosphate, sulf...

Large public water utilities currently are required to take several 100 mL water samples every month in their distribution systems and to test these samples for the presence of total coliforms to comply with the Total Coliform Rule. How and where they take these samples is not specified. The Total Coliform Rule is currently undergoing review and revision. One possible revision is more specific ...

The Colilert (CL) and Coliquik (CQ) systems were compared in a presence-absence format against the Standard Methods membrane filtration (MF) technique to determine whether differences existed in total coliform detection. Approximately 750 water samples were collected from distribution systems, covered and uncovered storage reservoirs, well sites, and the influent to drinking water treatment pla...

BACKGROUND Whereas street vended foods are readily available sources of meals for many people across the world, the microbial safety of such food is always uncertain. In developing countries the major sources of food-borne illnesses are street vended foods. The aim of this study was thus to assess the prevalence and antibiogram of bacteria from white lupin in Bahir Dar Town. METHODS A total o...

A new radiometric method for the detection of coliform bacteria in water has been described. The method is based on the release of (14)CO(2) from [(14)C]lactose by bacteria suspended in growth medium and incubated at 37 C. The evolved (14)CO(2) is trapped by hyamine hydroxide and counted in a liquid scintillation spectrometer. The method permits the detection of 1 to 10 organisms within 6 h of ...

In their study of the routine use of transcutaneous bilirubin (TcB) measurements in 27 well infant nurseries, Taylor et al found that 2.2% of TcB measurements underestimated the clinical laboratory measurement of total serum bilirubin (TSB) by $3.0 mg/dL, and the tendency for TcB to underestimate TSB increased with advancing newborn age (presumably up to the length of stay of a normal newborn)....

That some strains of the coliform group of bacteria exert inhibitory effects on other microorganisms, when grown in association in liquid media, was reported first by Nissle in 1916. Because of the multiplicity of factors involved when organisms are growing in heterogeneous mixtures in liquid media, the nature of the inhibiting agent is difficult to ascertain. Gratia of Belgium (1925) observed ...

Milk in Tanzania has been reported to be contaminated with large number of bacteria. This is because (1) milk is obtained from animals with unknown health status, (2) good milking and handling practices are to a large extent not observed, and (3) marketing and distribution are done in informal channels. These factors are potential causes of milk-borne diseases and milk quality loss. The aim of ...