Objective Thyroid cancer (TC) is one of the fastest-growing malignant tumors. This study sought to explore mechanism immune escape mediated by receptor tyrosine kinase (KIT) in TC. Methods The expression microarray TC was acquired through GEO database, and difference analysis Kyoto encyclopedia genes genomes pathway enrichment were carried out. KIT levels cell lines (K1/SW579/BCPAP) human norma...

Malaria threatens millions of people annually and is a burden to human health and economic development. Unfortunately in terms of disease control, no effective vaccines are available and the efficacy of treatment is limited by drug resistance. Genetic manipulation in Plasmodium falciparum is hampered due to the absence of robust methods for genetic analyses. Electroporation-based transfection m...

Purpose: To determine the effect of targeted silencing CDX2 with siRNA on vincristine-induced inhibition proliferation and apoptosis induction in leukemia K562 cells.
 Methods: cells were divided into untreated group, vincristine blank CDX2-siRNA group. The expression gene after transfection was determined by reverse-transcription-polymerase chain reaction (RT-PCR) immunoblotting, while MT...

Induction of protein synthesis by the external delivery of in-vitro transcription-messenger RNA (IVT-mRNA) has been a useful approach in the realm of cell biology, disease treatment, ‎reprogramming of cells, and vaccine design. Therefore, the development of new formulations for ‎protection of mRNA against nucleases is required to maintain its activity in-vivo. It was the aim o...

Induction of protein synthesis by the external delivery of in-vitro transcription-messenger RNA (IVT-mRNA) has been a useful approach in the realm of cell biology, disease treatment, ‎reprogramming of cells, and vaccine design. Therefore, the development of new formulations for ‎protection of mRNA against nucleases is required to maintain its activity in-vivo. It was the aim o...

OBJECTIVE To evaluate changes of nuclear factor-kappa B (NF-κB) during radioiodine 131 ((131)I) therapy and whether NF-κB inhibition could enhance (131)I-induced apoptosis in differentiated thyroid cancer (DTC) cells in a synergistic manner. METHODS Three human DTC cell lines were used. NF-κB inhibition was achieved by using a NF-κB inhibitor (Bay 11-7082) or by p65 siRNA transfection. Methyl...

OBJECTIVE Gastric cancer remains a worldwide burden as a leading cause of cancer-related death. Drug resistance of chemotherapy looms as a major clinical challenge to effective treatment. Recent research data has suggested that microRNAs could be a potential gastric cancer treatment strategy. To further evaluate the role of microRNAs on gastric cancer cells and its underlying possible mechanism...

Diagnostic ultrasound (US) is safe. Short interference (si) RNA of Frizzled (Fz)-9 suppresses proliferation of hepatocellular carcinoma cells. siRNA was introduced into Hep3B cells, a human hepatocellular carcinoma cell line, with transfection or US upward to culture plates at mechanical index (MI) = 0.4 or 0.8. siRNA of Fz9 was introduced for wes...

We have studied the molecular mechanism and signal transduction of pim-1, an oncogene encoding a serine-threonine kinase. This is a true oncogene which prolongs survival and inhibits apoptosis of hematopoietic cells. In order to determine whether the effects of Pim-1 occur by regulation of the mitogen-activated protein kinase pathway, we used a transcriptional reporter assay by transient co-tra...