Validation of an ELISA for the diagnosis of human trichinellosis 1 2 Running title: Serodiagnosis of human trichinellosis 3 4 Maria Angeles Gómez-Morales, 1* Alessandra Ludovisi, 1 Marco Amati, 1 Simona Cherchi, 1 5 Patrizio Pezzotti, 2 and Edoardo Pozio 1 6 7 Department of Infectious, Parasitic and Immunomediated Diseases, Istituto Superiore di 8 Sanità, 1 and Agenzia di Sanità Pubblica, 2 Rom...

This document provides a uniform set of recommendations for the control of Trichinella at all levels (on the farm, at slaughter and in processed meats). These recommendations are based on the best scientific information available and represent the official position of the International Commission on Trichinellosis regarding acceptable control methods. These recommendations are subject to change...

In 2008, an outbreak of human trichinosis associated with ingestion of raw soft-shelled turtles was identified and investigated in Taiwan. The data suggested that patients were likely infected with Trichinella papuae.

Results of our studies using ELISA and competitive inhibition assay (CIA) tests fully confirmed the previously experienced trichinellosis and reflected persistent stimulation of antibody production due to the antigen release from Trichinella larvae, which had survived longer and undergone gradual destruction in the muscles. The studies proved that the tests complement each other, yielding conco...

C57BL/6 male mice with chronic trichinellosis, and age-matched uninfected control mice, were inoculated with B16 melanoma cells. Tumour development was inhibited in the infected animals. In the infected tumour-bearing mice, the tumor induction intervals were longer and the tumour size was subsequently smaller than in the control mice. Moreover, when the number of tumour cells in the inoculum wa...

Serum samples belonging to three outbreaks in Argentina (47 patients) taken at different times post-ingestion were analysed employing IIF and ELISA simultaneously. Results show that: a) the number of patients diagnosed by a unique technique, especially by ELISA (31 patients), was lower than the one obtained by the simultaneous use of both assays (38 patients); b) four patients out of the seven ...

Trichinellosis is an important and under-recognized food-borne zoonosis in Southeast Asia. After 30 years of no reports, a small outbreak was described in Central Lao PDR (Laos) in 2003. Here we report a large outbreak of at least 650 estimated patients in Udomxay (northern Laos) in June 2005. Trichinella ELISA assays on serum from 133 patients and Western blot assays on 16 patients were positi...

The first human case with trichinellosis was reported in 1964 in Tibet, China. However, up to the present, the etiological agent of trichinellosis has been unclear. The aim of this study was to identify a Tibet Trichinella isolate at a species level by PCR-based methods. Multiplex PCR revealed amplicon of the expected size (173 bp) for Trichinella spiralis in assays containing larval DNA from T...