BACKGROUND At present, plant molecular systematics and DNA barcoding techniques rely heavily on the use of chloroplast gene sequences. Because of the relatively low evolutionary rates of chloroplast genes, there are very few choices suitable for molecular studies on angiosperms at low taxonomic levels, and for DNA barcoding of species. METHODOLOGY/PRINCIPAL FINDINGS We scanned the entire chlo...

BACKGROUND The morphological identification of different Hippophae species (Shaji) was difficult. This study aims to discriminate between medicinal and non-medicinal Hippophae species by DNA barcodes, the ITS2, psbA-trnH, and a combination of ITS2 and psbA-trnH (ITS2 + psbA-trnH). METHODS DNA was extracted from the dried fruit samples. Primer pairs ITS2F/3R for ITS2 and psbAF/trnHR for psbA-t...

Nepeta nuda L. (Lamiaceae) is a medicinal plant with wide distribution in Europa and Asia. In Bulgaria, N. also known as “naked (or hairless) catmint”, which likely refers to the naked or sparse short hairy stem leaves. This study aims generate DNA barcodes for precise genetic discrimination determination of phylogenetic position among other species. To achieve this goal, we applied barcoding t...

Nhằm hỗ trợ bảo tồn đa dạng tài nguyên thực vật, việc phát triển định danh tên khoa học và xác mối quan hệ di truyền của các loài vật là cần thiết nên được ưu tiên hiện cho sự bền vững. Chi Melodinus phân bố rộng rãi ở vùng nhiệt đới cận đới. Do tiềm năng về dược tính chúng mà chi này đã tâm khai thác, do đó nó cung cấp một ví dụ điển hình áp dụng nghiên cứu nhằm phụ vụ công tác thác nguyên. Ng...

DNA barcoding has been proposed to be one of the most promising tools for accurate and rapid identification of taxa. However, few publications have evaluated the efficiency of DNA barcoding for the large genera of flowering plants. Dendrobium, one of the largest genera of flowering plants, contains many species that are important in horticulture, medicine and biodiversity conservation. Besides,...

Quaternary climate and environment oscillations have profoundly shaped the population dynamic history geographic distributions of current plants. However, how endangered rare tree species respond to climatic environmental fluctuations in subtropical regions China East Asia still needs elucidation. In this study, we collected 36 natural populations an Magnolia sprengeri Pamp. determine demograph...

Piper species are used for spices, in traditional and processed forms of medicines, in cosmetic compounds, in cultural activities and insecticides. Here barcode analysis was performed for identification of plant parts, young plants and modified forms of plants. Thirty-six Piper species were collected and the three barcode regions, matK, rbcL and psbA-trnH spacer, were amplified, sequenced and a...

Pedicularis section Cyathophora is a monophyletic group characterized by perfoliate leaf and/or bract bases at each node. This section comprises four series, corresponding to four general corolla types of Pedicularis, i.e. toothless, toothed, beaked and long-tubed corollas. In this study, we aim to reconstruct a comprehensive phylogeny of section Cyathophora, and compare phylogenetic incongruen...

Brassicaceae is a large plant family of special interest; it includes many economically important crops, herbs, and ornamentals, as well as model organisms. The taxonomy of the Brassicaceae has long been controversial because of the poorly delimited generic boundaries and artificially circumscribed tribes. Despite great effort to delimitate species and reconstruct the phylogeny of Brassicaceae,...

The species within the Lolium/Festuca grass complex have dispersed and colonized large areas of temperate global grasslands both naturally and by human intervention. The species within this grass complex represent some of the most important grass species both for amenity and agricultural use worldwide. There has been renewed interest by grass breeders in producing hybrid combinations between th...