The ribosomal L12 protein gene of Sulfolobus solfataricus (SsoL12) has been subcloned and overexpressed in Escherichia coli. Five protein L12 mutants were designed: two NH2-terminal and two COOH-terminal truncated mutants and one mutant lacking the highly charged part of the COOH-terminal region. The mutant protein genes were overexpressed in E. coli and the products purified. The amino acid co...

A girl with congenital agammaglobulinemia and minor facial anomalies lacked B cells in peripheral blood: karyotypic analysis of white blood cells showed balanced translocation, t(9;20)(q33.2;q12). In the current study, we isolated a novel gene, leucine-rich repeat-containing 8 (LRRC8), at the translocation site on chromosome 9. It has four transmembrane helices with one isolated and eight seque...

Light signaling via the phytochrome A (phyA) photoreceptor controls basic plant developmental processes including de-etiolation and hypocotyl elongation. We have identified a new Arabidopsis mutant, pat (phytochrome A signal transduction)1-1, which shows strongly reduced responses in continuous far-red light. Physiological and molecular data indicate that this mutant is disrupted at an early st...

The aim of this study was preparation a soybean protein hydrolyzate with thrombin inhibitory activity and isolation, identification, characterization the active peptides from hydrolyzate. In study, inhibiting IC50 value 2.78 mg/mL prepared under improved pepsin hydrolysis condition, which exhibited highest in these protein-derived hydrolyzates. A novel nonapeptide FFPDIPKIK 2.36 mM screened by ...

GHRH is a hypothalamic peptide that stimulates the synthesis and secretion of GH from pituitary somatotroph cells. The GHRH receptor is a seven-transmembrane G protein-coupled receptor that localizes to the surface of somatotroph cells and binds GHRH. Alternative splicing of the GHRH receptor primary transcript at the intron/exon boundary 3' of exon 11 results in inclusion of sequence that is n...

BACKGROUND Hepatitis E Virus (HEV) is the causative agent of enterically transmitted acute hepatitis and has high mortality rate of up to 30% among pregnant women. Therefore, development of a novel vaccine is a desirable goal. OBJECTIVES The aim of this study was to construct tPAsp-PADRE-truncated open reading frame 2 (ORF2) and truncated ORF2 DNA plasmid, which can assist future studies with...

Problems concerning complete structural characterization of quasicrystals involve locating the atomic positions as well as determining the distribution of elements at each site. Quasicrystalline approximants provide models for potential building units of quasicrystals, but a clear determination of the elemental decorations in such approximants also remains incomplete. We report experimental and...

This paper explores the link between the presence of unions in the workplace, the adoption of decentralized labor agreements and technical efficiency, using a large sample of Italian manufacturing firms. We apply the Data Envelopment Analysis, and its robust version based on bootstrap theory, to get reliable estimates of technical efficiency at the firm level in a standard first stage. We devot...

The first part of this paper is concerned with global characterizations of both the multivariate ß-spline and the multivariate truncated power function as smooth piecewise polynomials. In the second part of the paper we establish combinatorial criteria for the linear independence of multivariate ß-splines corresponding to certain configurations of knot sets.