The N-methyl-D-aspartate (NMDA) subtype of glutamate receptor mediates fast excitatory neurotransmission, and agents that attenuate this function are neuroprotective, anesthetic, and psychotropic. To determine whether cAMP regulatable transcription factors play a role in the neurochemical actions of agents acting through NMDA receptors, the effects of the acute administration of uncompetitive a...

Androgens play a fundamental role in the morbidity and mortality of COVID-19, inducing both ACE-2 receptor to which SARS-CoV-2 binds gain entry into cell, TMPRS22, transmembrane protease that primes viral spike protein for efficient infection. The United States stands alone among developed nations permitting one androgen, oral DHEA, be freely available OTC online as ‘dietary supplement’. DHEA i...

In the July 2003 issue of Drug Metabolism and Disposition, an article on the conduct of in vitro and in vivo drug-drug interaction studies by the Pharmaceutical Research and Manufacturers of America (PhRMA) Drug Metabolism and Clinical Pharmacology Technical Working Groups (Bjornsson et al., 2003) was published. The inhibition of in vitro cytochrome P450 (P450) enzyme activity by a new molecula...

We provide initial rate data from enzymatic reaction experiments and tis processing to estimate the kinetic parameters from the substrate uncompetitive inhibition equation using the median method published by Eisenthal and Cornish-Bowden (Cornish-Bowden and Eisenthal, 1974; Eisenthal and Cornish-Bowden, 1974). The method was denominated the direct linear plot and consists in the calculation of ...

We estimate the effects of hospital competition on the level of and the variation in quality of care and hospital expenditures for elderly Medicare beneficiaries with heart attack. We compare competition’s effects on more-severely ill patients, whom we assume value quality more highly, to the effects on lessseverely ill, low-valuation patients. We find that low-valuation patients in competitive...

This study was designed to investigate the effects of molluscicidal components of Myristica fragrans Houtt. (Myristicaceae) on certain enzymes in the nervous tissue of freshwater snail Lymnaea acuminata Lamarck (Lymnaeidae). In vivo and in vitro treatments of trimyristin and myristicin (active molluscicidal components of Myristica fragrans Houtt.) significantly inhibited the acetylcholinesteras...

The catabolism of fungal 4-aminobutyrate (GABA) occurs via succinic semialdehyde (SSA). Succinic semialdehyde dehydrogenase (SSADH) from the acidogenic fungus Aspergillus niger was purified from GABA grown mycelia to the highest specific activity of 277 nmol min(-1) mg(-1), using phenyl Sepharose and DEAE Sephacel chromatography. The purified enzyme was specific for its substrates SSA and NAD+....

The inhibitory effects of trivalent and other metal ions on f erroxidase (ceruloplasmin EC 1.12.3.1.) activity were investigated. All trivalent cations tested inhibited ferroxidase activity but the strongest inhibitors had an ionic radius of 0.81 A or less. The inhibition by Al(III) was found to be mixed competitive and uncompetitive with respect to the substrate, Fe(I1). The uncompetitive feat...