Understanding the forces driving protein folding and aggregation is an essential step in developing means for controlling these important processes. Amide hydrogen exchange, coupled with mass spectrometry, has become an important method for studying protein unfolding and refolding. To extend procedures developed to study unfolding of relatively soluble proteins to less soluble, aggregation-pron...

Molecular dynamics simulations of protein folding and unfolding are often carried out at temperatures (400-600 K) that are much higher than physiological or room temperature to speed up the (un)folding process. Use of such high temperatures changes both the protein and solvent properties considerably, compared to physiological or room temperature. Water models designed for use in conjunction wi...

The capabilities of contemporary differential scanning and isothermal titration microcalorimetry for studying the thermodynamics of protein unfolding/refolding and their association with partners, particularly target DNA duplexes, are considered. It is shown that the predenaturational changes of proteins must not be ignored in studying the thermodynamics of formation of their native structure a...

The class of 2-automatic paperfolding sequences corresponds to the class of ultimately periodic sequences of unfolding instructions. We rst show that a paper-folding sequence is automatic ii it is 2-automatic. Then we provide families of minimal nite-state automata, minimal uniform tag sequences and minimal substitutions describing automatic paperfolding sequences, as well as a family of algebr...

Clinical doctorates are emerging in occupational therapy. By examining the development of clinical doctorates in medicine, dentistry, psychology, pharmacy, nursing, and physical therapy, implications can be drawn for the future of occupational therapy education. These histories offer us a sense of the distinct purpose and curricular structure of the professional doctorate, its successes and pro...

Loops are abundant in native RNA structures and proliferate close to the unfolding transition. By including a statistical weight approximately l(-c) for loops of length l in the recursion relation for the partition function, we show that the heat capacity depends sensitively on the presence and value of the exponent c, even for a short explicit tRNA sequence. For long homo-RNA, we analytically ...

While evidence is mounting that cells exploit protein unfolding for mechanochemical signal conversion (mechanotransduction), what mechanisms are in place to deal with the unwanted consequences of exposing hydrophobic residues upon force-induced protein unfolding? Here, we show that mechanical chaperones exist that can transiently bind to hydrophobic residues that are freshly exposed by mechanic...

The mechanical unfolding of a set of 12 proteins with diverse topologies is investigated using an all-atom constraint-based model. Proteins are represented as polypeptides cross-linked by hydrogen bonds, salt bridges, and hydrophobic contacts, each modeled as a harmonic inequality constraint capable of supporting a finite load before breaking. Stereochemically acceptable unfolding pathways are ...

We study the relationship between the Mosco convergence of a sequence of convex proper lower semicontinuous functionals, defined on a reflexive Banach space, and the convergence of their subdifferentiels as maximal monotone graphs. We then apply these results together with the unfolding method (see [10]) to study the homogenization of equations of the form − div dε = f , with (∇uε(x), dε(x)) ∈ ...