This study evaluated the effects of blood contamination and decontamination methods during different steps of bonding procedures on the microtensile bond strength of two-step self-etch adhesives to dentin. Sixty extracted human molars were ground flat to expose occlusal dentin. The 60 molars were randomly assigned to three groups, each treated with a different two-step self-etch adhesive: Clear...

This is an open access article distributed under the terms of the Creative Commons Attribution License (CC-BY-NC-SA 4.0), which permits unrestricted noncommercial use and redistribution provided that the original author and source are credited and the new work is distributed under the same license as the original. British whitebeams (Sorbus aria aggr.; Rosaceae) are an em-blematic case study of...

Despite much progress over the past decade, current Single Nucleotide Polymorphism (SNP) genotyping technologies still offer an insufficient degree of multiplexing when required to handle user-selected sets of SNPs. In this paper we propose a new genotyping assay architecture combining multiplexed solution-phase single-base extension (SBE) reactions with sequencing by hybridization (SBH) using ...

The method of differential display developed by Liang and Pardee (10) has gained a large audience and is now the method of choice to quickly identify and isolate differentially expressed genes in several experimental systems. The method is powerful because of its inherent simplicity, and it allows the simultaneous comparison of upand down-regulated genes with small amounts of raw material. Neve...

In this study polymerase chain reaction (PCR) was used to identify yeast in domestic ragi obtained from two local markets in Sarawak and Pahang. These ragi are normally used as a dry starter in food fermentation (tapai) for Pahang (ST2) and Sarawak (ST3) and tuak (ST1) which is an alcoholic drink in Sarawak. Universal primer, NL1 and NL4 were used as a primer in this study to amplify D1/D2 frag...

Sequence tagged microsatellite profiling (STMP) enables the rapid development of large numbers of co-dominant DNA markers, known as sequence tagged microsatellites (STMs). Each STM is amplified by PCR using a single primer specific to the conserved DNA sequence flanking the microsatellite repeat in combination with a universal primer that anchors to the 5'-ends of the microsatellites. It is als...

http://bfw.ac.at/200/1859.html The data collected: A data base is presented which collects published primer information for chloroplast DNA. Additional primers were designed in order to fill gaps were no or little primer information could be found. Chloroplast genes evolve slowly, and many primers have been designed to work across species ('universal primers', e.g. Taberlet et al. 1991, Grivet ...

Insect mitochondrial genome (mtDNA) analysis is a powerful tool for the study of population genetics and phylogenetics. In the past few years primer sequences for the PCR amplification of various insect mtDNA genes have been published. The objectives of this study were (1) present new primer sequences for six insect mitochondrial genes and (2) test primers designed in our laboratory and some pr...

Streptococcus mutans have been proposed as the main causative agent of dental cavities. Dental caries is among the most common diseases in the world, this study was done to determine whether PCR analysis is useful to identify and even classify oral and other streptococci. 20 dental plaque samples were collected from teeth, 10 normal sample and 10 from persons with oral problems. The samples wer...

C-type lectin-like proteins (CTLPs) isolated from snake venoms are the largest and most complex non-mammalian vertebrate C-type lectin-like domain family. In the present study, we simultaneously amplified four cDNAs encoding different types of CTLP subunits from the venoms of two different species of snakes by RT-PCR with a single sense primer and a nested universal primer - two CTLP subunit-en...