Multiple sequence alignment is increasingly important to bioinformatics, with several applications ranging from phylogenetic analyses to domain identification. There are several ways to perform multiple sequence alignment, an important way of which is the progressive alignment approach studied in this work. Progressive alignment involves three steps: find the distance between each pair of seque...

This exploratory study determined the epidemiological characteristics of Staphylococcus epidermidis from near touch sites and blood cultures in one Intensive Care Unit (ICU) between July and October 2009 in a large teaching hospital in Brazil. Five different touch sites around the environment of two different patients were sampled over four days and 24 blood cultures from 24 patients were obtai...

In the present study, RAPD-PCR was used to assess genetic diversity of the rye including landrances and new rye cultivars coming from Central Europe and the Union of Soviet Socialist Republics (SUN). Five arbitrary random primers were used to determine RAPD polymorphism in the set of 38 rye genotypes. These primers amplified altogether 43 different DNA fragments with an average number of 8.6 fr...

Genetic diversity of 12 lemon balm ecotypes was evaluated using agronomic traits as well as ISSR and RAPD markers. Results of analysis of variance showed high diversity among the studied ecotypes. Cluster analysis using UPGMA method grouped the ecotypes into three different groups based on the agronomic traits. Twelve ISSR primers created 106 polymorphic bands among the studied ecotypes. An ISS...

Some Trichoderma isolates were collected from different locations of Bangladesh for evaluating their bioefficiency by determining their genetic variations. PCR-based Random Amplified Polymorphic DNA (RAPD) Marker employing 3 decamer primers produced 29 scorable bands of which all (100%) were polymorphic. The co-efficient of gene differentiation (Gst) was 1.0000 reflecting the existence of high ...

An experiment was conducted to analyze the genetic diversity among 38 maize germplasm accessions of the maize germplasm bank of Department of Millets, TNAU, Coimbatore. Observations regarding 25 morphological traits were recorded and the data matrix was used for estimation of Euclidian distance by Un-weighted Paired Group Arithmetic Average (UPGMA) method. Clustering was done by Sequential Aggl...

Two major religious groups of India were studied, viz. Hindu and Muslim. The survey was confined to 1059 individuals of Aligarh city (UP). Households were selected on random a priori basis during day time by door to door contact. Muslims populations were Syed, Sheikh, Pathan, Ansari and Hindus were Brahmin, Bania, Rajput, Jatav. Gene frequency, allele frequency, heterozygosity, genetic identity...

The DNA polymorphism among 92 selected tef genotypes belonging to eight origin groups was assessed using eight inter simple sequence repeat (ISSR) primers. The objectives were to examine the possibility of using ISSR markers for unravelling genetic diversity in tef, and to assess the extent and pattern of genetic diversity in the test germplasm with respect to origin groups. The eight primers w...

The study was carried out to analyze the genetic diversity of five maize varieties at K. Wagh collage agricultural Biotechnology, Nashik during May October 2022 Genomic DNA isolated from by using CTAB method. PCR amplification obtains with set SSR primers. dendrogram constructed based UPGMA method Jaccard’s similarity coefficient and subjected cluster analysis. Among primer used only three foun...

This study aims to characterize different Salmonella enterica subsp molecularly. strains (n=49) were isolated from human gastrointestinal cases in the Tolima region and poultry Santander regions using PCR-RFLP, PCR-ribotyping, PCR-SSCP. The band patterns obtained with each technique analyzed by building dendrograms based on Unweighted Pair Group Method Arithmetic mean (UPGMA) method Dice coeffi...