The aim of the present study was to examine the signaling pathways for a low dose of angiotensin II (ANG II) on Na+ uptake of primary cultured rabbit renal proximal tubule cells (PTCs) in hormonally defined serum-free medium. The results were as follows; ANG II (10(-11) M) stimulated the proliferation of PTCs. 10(-11) M ANG II stimulated Na+ uptake by 20%, whereas 10(-9) M ANG II inhibited it b...

The mechanism of bacterial uptake of vitamin B(12), the spectrum of microorganisms capable of such uptake, and the factors involved were the subject of this study. Bacterial uptake of vitamin B(12) was found to be at least a two stage process. A primary uptake phase which was rapid (1 min or less), pH dependent, nontemperature dependent, did not require viable organisms and was insensitive to e...

BACKGROUND Glucose homeostasis is maintained by a balance between hepatic glucose production and peripheral glucose utilization. In skeletal muscle cells, glucose utilization is primarily regulated by glucose uptake. Deprivation of cellular energy induces the activation of regulatory proteins and thus glucose uptake. AMP-activated protein kinase (AMPK) is known to play a significant role in the...

rosemary (rosmarinus officinalis) is a common household plant grows in many parts of the world, including iran. rosemary leaves are used in folk medicine as an antispasmodic, analgesic, diuretic and ntiepileptic agent. the objective of this study was to investigate the antinociceptive mechanisms of rosemary extract using a writhing test as a model of visceral pain. possible antinociceptive mech...

K+ is a competitive inhibitor of the uptake of the other alkali metal cations by yeast. Rb+ is a competitive inhibitor of K+ uptake, but Li+, Na+, and Cs+ act like H+. At relatively low concentrations they behave as apparent noncompetitive inhibitors of K+ transport, but the inhibition is incomplete. At higher concentrations they inhibit the remaining K+ transport competitively. Ca++ and Mg++ i...

Ruthenium red is a well known inhibitor of Ca2+ uptake into mitochondria in vitro. However, its utility as an inhibitor of Ca2+ uptake into mitochondria in vivo or in situ in intact cells is limited because of its inhibitory effects on sarcoplasmic reticulum Ca2+ release channel and other cellular processes. We have synthesized a ruthenium derivative and found it to be an oxygen-bridged dinucle...

An investigation was made of azaserine entry into cells of a sensitive and resistant line of the plasma-cell neoplasm 70429. Transport experiments determined (a) the initial velocity of concentrative uptake in the first 1 or 2 minutes of exposure to the compound, (6) the total kinetics of uptake over 30-minute to 90-minute periods, and (c) the dependence of steady-state uptake on the extracellu...

Abstract Arsenic (As) negatively affects plant development. This study evaluates how the application of silicon (Si) can favor formation adventitious roots in rice under arsenate stress (AsV) as a mechanism to mitigate its negative effects. The simultaneous AsV and Si up-regulated expression genes involved nitric oxide (NO) metabolism, cell cycle progression, auxin (IAA, indole-3-acetic acid) b...