نتایج جستجو برای: using commercial kit and rnease mini kit
تعداد نتایج: 17345105 فیلتر نتایج به سال:
Article Type: Full Length Research Article Polymerase chain reaction (PCR) and loop mediated isothermal amplification (LAMP) represent a major advance in diagnostic methods in terms of speed and sensitivity. However, standard methods for sample preparation prior to PCR and LAMP are laborious and time consuming. Three template preparation methods (crude template, boiling, and commercial DNA extr...
Background: There are accumulating evidences showing the role of adiponectin, a newly discovered adipocytokine secreted from adipose tissue, in the regulation of the ovarian function in dairy cows. The objective of the present study was to evaluate the pattern of serum adiponectin concentration during a normal estrous cycle in the postpartum dairy cows. Materials and Methods: Coccygeal vein blo...
Three widely used commercial hemoglobin electrophoresis kits were studied to ascertain specificity, accuracy, precision, and characteristics of product information. Two hundred and ninety-six hemoglobin samples were used to evaluate each kit. Except for poor separation of hemoglobin F by one kit, all three kits gave generally satisfactory results. We give specific recommendations for improving ...
A novel commercial Chikungunya virus real-time reverse transcription-PCR (RT-PCR) kit was evaluated on a comprehensive panel of original patient samples. The assay was 100% sensitive and specific in comparison to a published real-time RT-PCR. Viral loads from both assays were highly correlated. The kit proved to be suitable for routine use in patient care.
background: bradford protein assay is popular due to its ease of performance and relative sensitivity. many researchers and laboratories in iran use standard assay of bradford by cuvette. no commercial kit was available for bradford microplate assay in iran. meanwhile, imported bradford commercial kits are very expensive and have a long delivery time in iran. till now no study or document on br...
Abstract Equine infectious anemia is a worldwide distributed disease that affects the Equide family. Commercial effective vaccine not available, for reason control of depends on diagnostic tools. To improve efficiency program in Cuba, LABIOFAM Group, developed an indirect enzyme-linked immunosorbent assay (ELISA), ELISA kit, to complement system currently uses agar gel immunodiffusion (AGID) ki...
OBJECTIVE We report here the human leukocyte antigen (HLA) allelic variety and haplotype composition in a cohort of the Taiwanese Chinese population and their patterns of linkage disequilibria on HLA-B: HLA-C alleles and HLA-DRB1: HLA-DQB1 alleles at a high-resolution level. MATERIALS AND METHODS Peripheral whole blood from 11,423 Taiwanese Chinese unrelated individuals was collected in acid ...
This report describes the histological and immunohistochemical features of a small intestinal tumor that resembled a human gastrointestinal stromal tumor (GISTs) in a seven-year-old male cross-breed dog. This was unique because of the expression of desmin by the tumor. Grossly, the white-gray tumor measured 8.0 × 4.0 × 6.5 cm and was 760 gram in weight. It was cystic and enveloped the jejunum. ...
This study aimed to test different protocols for the extraction of microbial DNA from the coral Mussismilia harttii. Four different commercial kits were tested, three of them based on methods for DNA extraction from soil (FastDNA SPIN Kit for soil, MP Bio, PowerSoil DNA Isolation Kit, MoBio, and ZR Soil Microbe DNA Kit, Zymo Research) and one kit for DNA extraction from plants (UltraClean Plant...
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