[reaction: see text] The synthesis of 2'-deoxycytidine nucleosides bearing amino and thiol groups appended to the 5-position of the nucleobase via a butynyl linker is described. The corresponding triphosphates were then synthesized from the nucleoside and incorporated into oligonucleotides by Vent (exo(-)) DNA polymerase. The ability of Vent (exo(-)) polymerase to amplify oligonucleotides conta...

Citation: Boschen RE, Rowden AA, Clark MR and Gardner JPA (2015) Limitations in the Use of Archived Vent Mussel Samples to Assess Genetic Connectivity Among Seafloor Massive Sulfide Deposits: A Case Study with Implications for Environmental Management. Front. Mar. Sci. 2:105. doi: 10.3389/fmars.2015.00105 Limitations in the Use of Archived Vent Mussel Samples to Assess Genetic Connectivity Amon...

Recent evidence suggests that deep-sea vestimentiferan tube worms acquire their endosymbiotic bacteria from the environment each generation; thus, free-living symbionts should exist. Here, free-living tube worm symbiont phylotypes were detected in vent seawater and in biofilms at multiple deep-sea vent habitats by PCR amplification, DNA sequence analysis, and fluorescence in situ hybridization....

Two hydroxyethylamine chroman derivatives, ammonificins A (1) and B (2), were isolated from the marine hydrothermal vent bacterium Thermovibrio ammonificans. The molecular structures of these compounds were determined using a combination of NMR, mass spectrometry, and CD analyses. Biological activities were determined using an antimicrobial assay and the patented ApopScreen cell-based screen fo...

BACKGROUND The speciation of dissolved sulfide in the water immediately surrounding deep-ocean hydrothermal vents is critical to chemoautotrophic organisms that are the primary producers of these ecosystems. The objective of this research was to identify the role of Zn and Fe for controlling the speciation of sulfide in the hydrothermal vent fields at the Eastern Lau Spreading Center (ELSC) in ...

China's Chang'e-5 (CE-5) mission recently returned samples from a young intermediate-Ti mare unit (Em4/P58, ∼1.5 Ga) in Northern Oceanus Procellarum. Rima Sharp, previously mapped as the longest lunar sinuous rille, is most prominent volcanic feature associated with landing region. Our analysis shows that Sharp not single but instead composed of two separate rilles (Rima originating North Vent,...

Site U1328 (Scientific Prospectus Site CAS-06A) is located within a seafloor cold-vent field, with dimensions 2 km by 4 km, consisting of at least four vents associated with nearsurface faults. The cold vents are characterized by near-vertical seismic blank (or wipe-out) zones that are between 80 and several 100 m wide, and show a clear E-W trend as identified from 3D seismic imaging. The most ...

As part of an ongoing examination of microbial diversity associated with hydrothermal vent polychaetes of the family Alvinellidae, we undertook a culture-independent molecular analysis of the bacterial assemblage associated with mucous secretions of the Northeastern Pacific vent polychaete Paralvinella palmiformis. Using a molecular 16S rDNA-based phylogenetic approach, clone libraries were con...

During a previously published study on gaseous microemboli (GMEs) and perfusionist interventions, it was noted that emboli could be detected after the arterial filter when blood/air challenges entered the membrane oxygenator's integral cardiotomy. The findings indicated that further study into the oxygenator's integral cardiotomy reservoir was warranted. This is the first know published report ...