نتایج جستجو برای: vhh
تعداد نتایج: 516 فیلتر نتایج به سال:
Abstract TRAP-transporters are widespread in bacteria and archaea often responsible for the transport of nutrients or virulence factors pathogens. They consist three domains, a substrate-binding protein two membrane domains. Using cryo-EM, we have determined structure revealing monomeric elevator architecture. Further studies gave insights into interaction between domains how they can be specif...
Hypertrophic differentiation occurs during in vitro chondrogenesis of mesenchymal stem cells (MSCs), decreasing the quality of the cartilage construct. Previously we identified WNT pathway antagonists Dickkopf 1 homolog (DKK1) and frizzled-related protein (FRZB) as key factors in blocking hypertrophic differentiation of human MSCs (hMSCs). In this study, we investigated the role of endogenously...
The important family of G protein-coupled receptors has so far not been targeted very successfully with conventional monoclonal antibodies. Here we report the isolation and characterization of functional VHH-based immunoglobulin single variable domains (or nanobodies) against the chemokine receptor CXCR4. Two highly selective monovalent nanobodies, 238D2 and 238D4, were obtained using a time-ef...
Chemical crop protection is widely used to control plant diseases. However, the adverse effects of pesticide use on human health and environment, resistance development and the impact of regulatory requirements on the crop protection market urges the agrochemical industry to explore innovative and alternative approaches. In that context, we demonstrate here the potential of camelid single domai...
Single-domain antibodies (sdAbs) found in camelids lack a light chain, and their antigen-binding site sits completely in the heavy-chain variable domain (VHH). Their simplicity, thermostability, and ease in expression have made VHHs highly attractive. Although this has been successfully exploited for macromolecular antigens, their application to the detection of small molecules is still limited...
Oculopharyngeal muscular dystrophy (OPMD) belongs to the group of protein aggregation disorders and is caused by extensions of the N-terminal polyalanine stretch of the nuclear polyA-binding protein 1 (PABPN1). The presence of PABPN1-containing intranuclear aggregates in skeletal muscle is unique for OPMD and is also observed in transgenic mouse and cell models for OPMD. These models consistent...
JJX12 is an engineered bispecific antibody against ricin, a member of the medically important A-B family of toxins that exploits retrograde transport as means to gain entry into the cytosol of target cells. JJX12 consists of RTA-D10, a camelid single variable domain (VHH) antibody directed against an epitope on ricin's enzymatic subunit (RTA), linked via a 15-mer peptide to RTB-B7, a VHH agains...
با توجه به کاربردهای گسترده تشخیصی و درمانی آنتی بادیهای مونوکلونال، تولید آنها از منابع دائمی، ایمن و ارزان حائز اهمیت می باشد. از جمله، می توان به آنتی بادی مونوکلونال تک دومنی با منشأ شتری vhh اشاره نمود که به دلیل دارا بودن ویژگیهایی از جمله: تشابه بسیار با زنجیره سنگین آنتی بادیهای انسانی، حلالیت، تمایل و اتصال اختصاصی به آنتی ژن، بر دیگر انواع آنتی بادیها ترجیح داده می شود. در این تحقیق ا...
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