This study documents that chlorinated analogs of leukotoxin diol 1, in which the vic-diol has been replaced with vic-chlorides (2), induce caspase 3 activity and apoptosis on HepG2 cells in a dose-dependent manner in analogy to the parent diol. This suggests that chlorides may substitute for hydroxyls in certain lipids as bioisosteres in defined biological settings.

Main Article: Reflection on the Development of a Research Agenda in Rural Health Lisa Bourke Department of Rural Health, The University of Melbourne PO Box 6500, Shepparton, VIC 3632, AUSTRALIA [email protected] James D. Best Lee Kong Chian School of Medicine Nanyang Technological University, Novena Campus 11 Mandalay Road, SINGAPORE 308232 [email protected] John Wakerman Faculty of Medi...

The regulation of phosphate (Pi) handling is crucial during calcification of the aortic valve. Gene profiling of Pi transporters revealed that VIC culture expresses SLC201A1/Pit1 and SLC20A2/Pit2. On exposure to a mineralizing medium (2 mM Pi), the expression of Pi transporters in VIC culture is increased several folds, with the highest magnitude for SLC20A1. By using siRNAs, we established tha...

Dustin J. Flanagan,1,2,10 Toby J. Phesse,3,10,11 Nick Barker,4,5,10 Renate H.M. Schwab,1,2 Nancy Amin,1,2 JordaneMalaterre,6 Daniel E. Stange,7 Cameron J. Nowell,3 Scott A. Currie,1,2 Jarel T.S. Saw,1,2 Eva Beuchert,1,2 Robert G. Ramsay,6 Owen J. Sansom,8 Matthias Ernst,3,11 Hans Clevers,7 and Elizabeth Vincan1,2,9,* 1Department of Anatomy and Neuroscience, University of Melbourne, Melbourne, V...

Heart valve disease is an important cause of morbidity and mortality worldwide. Little is known about valve disease pathogenesis, but increasing evidence implicates a genetic basis for valve disease, suggesting a developmental origin. Although the cellular and molecular processes involved in early valvulogenesis have been well described, less is known about the regulation of valve extracellular...

Monoclonal antibodies were produced against surface antigens of live cells from a human acute monocytic leukaemia cell line (THP-1). One clone, VIC-C2, when assayed by immunofluorescence microscopy, brightly stained the surface of THP-1 cells and the cytoplasm of Langerhans cells, fibroblasts and melanocytes in sections of human skin. The immunoreactive cytoplasmic structures were filamentous a...

AIMS Aortic valve stenosis (AS) is the most common valvulopathy and is characterized by inflammation, extracellular matrix (ECM) remodelling and calcification, causing a narrowing of the valve and the consequential obstruction of the cardiac outflow. Although intraleaflet haemorrhage is associated with AS progression, the mechanisms involved are not known. The aims of this study were to identif...